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An Immunochromatographic Lateral Flow Strip Test for the Rapid Detection of Danofloxacin in Milk

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Abstract

An immunochromatographic lateral flow strip test (ILFST) for the rapid detection of danofloxacin (DAN) in milk samples was developed based on colloidal gold-labeled anti-DAN monoclonal antibody 3E9 and a competitive format. Quantitative detections of DAN-spiked samples using the ILFST gave a half inhibitory concentration (IC50) of 0.513 ng/mL and a limit of detection (LOD) of 0.092 ng/mL. Recoveries of DAN from spiked milk samples were from 80.0 to 95.1% within an assay (intra-assay) and from 87.0 to 89.8% between assays (inter-assay). The coefficients of variation (CV) for intra-assay and inter-assay were 2.09–9.93% and 2.42–12.07%, respectively. These data indicated that the ILFST was highly sensitive and reproducible. Detections of authentic milk samples using ILFST and high-performance liquid chromatography (HPLC) showed no significant differences between the two methods. Hence, the ILFST can be widely used for both qualitative and quantitative detection of DAN residues in milk samples.

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Funding

This study was supported by the National Science & Technology Pillar Program of the 12th Five-Year Plan (Grant No. 2014BAD13B05), the Natural Science Foundation of Henan Province (Grant No. 182300410093), and Henan Province Science and Technology Agency (Grant No. 182102310683).

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Authors and Affiliations

  1. Institute of Food and Drug Inspection, Zhoukou Normal University, Zhoukou, 466001, People’s Republic of China

    Xingdong Yang, Zhongke Sun, Zonghao Yue, Lili Li & Le He

  2. Key Laboratory of Animal Immunology of the Ministry of Agriculture, Henan Provincial Key Laboratory of Animal Immunology, Henan Academy of Agricultural Sciences, Zhengzhou, 450002, People’s Republic of China

    Xingdong Yang, Jifei Yang & Xiaofei Hu

  3. School of Public Health, Xinxiang Medical University, Xinxiang, 453003, People’s Republic of China

    Yinbiao Wang

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  1. Xingdong Yang
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  4. Zhongke Sun
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  5. Zonghao Yue
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Correspondence to Xiaofei Hu.

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Xingdong Yang declares that he has no conflict of interest. Yinbiao Wang declares that he has no conflict of interest. Jifei Yang declares that he has no conflict of interest. Zhongke Sun Wang declares that he has no conflict of interest. Zonghao Yue declares that he has no conflict of interest. Lili Li declares that she has no conflict of interest. Le He declares that he has no conflict of interest. Xiaofei Hu declares that he has no conflict of interest.

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Yang, X., Wang, Y., Yang, J. et al. An Immunochromatographic Lateral Flow Strip Test for the Rapid Detection of Danofloxacin in Milk. Food Anal. Methods 12, 2430–2437 (2019). https://doi.org/10.1007/s12161-019-01601-9

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