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Amperometric Determination of Glucose in White Grape and in Tablets as Ingredient by Screen-Printed Electrode Modified with Glucose Oxidase and Composite of Platinum and Multiwalled Carbon Nanotubes

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Abstract

Multiwalled carbon nanotubes (MWCNTs) and nanocomposite made from MWCNTs and Pt nanoparticles (Pt-MWCNTs) were used as bulk modifiers of screen-printed carbon electrodes (SPCEs). Cyclic voltammetric and amperometric measurements were performed by the homemade substrate electrodes, the bare SPCE and the modified ones (MWCNT-SPCE and Pt-MWCNT-SPCE), with aim to investigate their applicability for determination of H2O2 in phosphate buffer solution (0.1 M; pH 7.50) as supporting electrolyte. Pt-MWCNT-SPCE showed improved analytical performances including the significant decrease in overpotential of H2O2 compared to SPCE and MWCNT-SPCE. So, Pt-MWCNT-SPCE served as substrate electrode for design of a simple first-generation biosensor made from glucose oxidase (GOx) and Nafion® forming the GOx/Pt-MWCNT-SPCE for reliable determination of glucose via its enzymatic by-product, the H2O2. The surface morphology characterization by scanning electron microscopy confirmed the presence of immobilized spherical shaped biosensing units at the GOx/Pt-MWCNT-SPCE. In the case of amperometry of glucose, optimal potential for the working electrode was − 0.50 V vs. SCE, and a satisfactory linearity was obtained in the tested concentration range from 65.8 to 260.6 μg mL−1, with estimated LOQ of 35.0 μg mL−1. The developed cost-effective amperometric method in combination with GOx/Pt-MWCNT-SPCE was successfully applied for the determination of glucose in selected food samples: white grapes and glucose tablets. The obtained results were in good agreement with those received by the alternatively used commercially available glucometer and by the producer declared value in the case of the tablet sample as well.

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Acknowledgements

GV thanks the Domus Hungarica Scientiarum et Artium fellowship.

Funding

This study received a financial support from the Ministry of Science and Technological Development of the Republic of Serbia (Project Nos. 172059 and 172012) and CEEPUSIII (CZ-0212-09-1718) network.

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Authors and Affiliations

  1. Faculty of Sciences, Department of Chemistry, Biochemistry and Environmental Protection, University of Novi Sad, Trg D. Obradovića 3, Novi Sad, 21000, Serbia

    Valéria Guzsvány, Jasmina Anojčić, Olga Vajdle & Emil Radulović

  2. Department of Applied and Environmental Chemistry, University of Szeged, Rerrich Béla tér 1, Szeged, 6720, Hungary

    Dániel Madarász & Zoltán Kónya

  3. MTA-SZTE Reaction Kinetics and Surface Chemistry Research Group, Rerrich Béla tér 1, Szeged, 6720, Hungary

    Zoltán Kónya

  4. Institute of Chemistry-Analytical Chemistry, Karl-Franzens University Graz, Universitaetsplatz 1, 8010, Graz, Austria

    Kurt Kalcher

Authors
  1. Valéria Guzsvány
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  2. Jasmina Anojčić
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  3. Olga Vajdle
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  4. Emil Radulović
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  5. Dániel Madarász
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  6. Zoltán Kónya
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  7. Kurt Kalcher
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All authors named in the manuscript are entitled to the authorship and have approved the final version of the submitted manuscript.

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Correspondence to Valéria Guzsvány.

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Conflict of Interest

Valéria Guzsvány declares that she has no conflict of interest. Jasmina Anojčić declares that she has no conflict of interest. Olga Vajdle declares that she has no conflict of interest. Emil Radulović declares that he has no conflict of interest. Dániel Madarász declares that he has no conflict of interest. Zoltán Kónya declares that he has no conflict of interest. Kurt Kalcher declares that he has no conflict of interest.

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This article does not contain any studies with human participants or animals performed by any of the authors. This is an original research article that has neither been published previously nor considered presently for publication elsewhere.

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Guzsvány, V., Anojčić, J., Vajdle, O. et al. Amperometric Determination of Glucose in White Grape and in Tablets as Ingredient by Screen-Printed Electrode Modified with Glucose Oxidase and Composite of Platinum and Multiwalled Carbon Nanotubes. Food Anal. Methods 12, 570–580 (2019). https://doi.org/10.1007/s12161-018-1387-7

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  • DOI: https://doi.org/10.1007/s12161-018-1387-7

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