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Screen-printed enzymatic glucose biosensor based on a composite made from multiwalled carbon nanotubes and palladium containing particles

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Abstract

A composite material consisting of multiwalled carbon nanotubes and palladium containing particles was synthesized and applied to the preparation of bulk-modified screen-printed carbon electrodes (Pd-MWCNT-SPCE) and surface-modified screen-printed carbon electrodes (Pd-MWCNT/SPCE). They were characterized by cyclic voltammetry and hydrodynamic chronoamperometry in solution of pH 7.5. Both electrodes were then modified with glucose oxidase (GOx) by drop-coating a solution of GOx and Nafion® on their surface. Glucose can be determined via enzymatically formed H2O2. In an alternative approach, gold nanoparticles (5 nm) were incorporated into the biolayer of the electrodes. The resulting electrodes of type GOx/Pd-MWCNT-SPCE and GOx-Au/Pd-MWCNT-SPCE showed acceptable analytical performance at working potentials between −0.20 V and −0.50 V in case of hydrodynamic chronoamperometry. Both electrodes can be operated best at a working potential of −0.40 V vs SCE, with acceptable linearity of the methods in sub mM concentration ranges and with LOQs of 0.14 mM and 0.07 mM for glucose for the GOx/Pd-MWCNT-SPCE and GOx-Au/Pd-MWCNT-SPCE, respectively. Incorporation of gold nanoparticles prolongs the operational lifetime of the electrodes by two weeks. The GOx/Pd-MWCNT-SPCE based method was applied to the determination of glucose in multifloral honey, and the GOx-Au/Pd-MWCNT-SPCE method to the determination of glucose in blood serum. In both cases there was a good agreement with the results obtained by commercially available equipment for determination of glucose.

Schematic of a screen printed carbon biosensor based on the use of multiwalled carbon nanotubes modified with palladium-containing particles and glucose oxidase. It can be applied to the amperometric determination of glucose in blood serum and multifloral honey

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Acknowledgements

The authors acknowledge the financial support of the Ministry of Science and Technological Development of the Republic of Serbia (Project Nos. 172059 and 172012), and CEEPUSIII (CZ-0212-09-1516) network, and for MATCROSS (HUSRB 1002/214/188) for the synthesis of MWCNT and Pd-MWCNT materials.

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Authors and Affiliations

  1. Faculty of Sciences, Department of Chemistry, Biochemistry and Environmental Protection, University of Novi Sad, Trg D. Obradovića 3, 21000, Novi Sad, Serbia

    Valéria Guzsvány, Jasmina Anojčić, Emil Radulović & Olga Vajdle

  2. Department of Chemistry, Dalian University of Technology, Linggong Road No. 2, Ganjingzi District, Dalian City, Liaoning Province, 116024, People’s Republic of China

    Igor Stanković

  3. Department of Applied and Environmental Chemistry, University of Szeged, Rerrich Béla tér 1, Szeged, 6720, Hungary

    Dániel Madarász & Zoltán Kónya

  4. MTA-SZTE Reaction Kinetics and Surface Chemistry Research Group, Rerrich Béla tér 1, Szeged, 6720, Hungary

    Zoltán Kónya

  5. Institute of Chemistry-Analytical Chemistry, Karl-Franzens University Graz, Universitaetsplatz 1, 8010, Graz, Austria

    Kurt Kalcher

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  1. Valéria Guzsvány
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  2. Jasmina Anojčić
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  3. Emil Radulović
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  4. Olga Vajdle
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  5. Igor Stanković
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  7. Zoltán Kónya
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Correspondence to Valéria Guzsvány.

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Guzsvány, V., Anojčić, J., Radulović, E. et al. Screen-printed enzymatic glucose biosensor based on a composite made from multiwalled carbon nanotubes and palladium containing particles. Microchim Acta 184, 1987–1996 (2017). https://doi.org/10.1007/s00604-017-2188-1

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