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Indirect Competitive Chemiluminescence Immunoassay for Glycosides Daidzin and Genistin in Soy Products

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Abstract

Daidzin (DZ) and genistin (GEN) are the main glucosidic components of soy isoflavone; the detection of DZ and GEN can provide insight for the appropriate ingestion of soy products. An indirect competitive chemiluminescence immunoassay (icCLIA) method based on N-(aminobutyl)-N-(ethylisoluminol) (ABEI) as chemiluminescent (CL) label was developed to assay DZ in soy products. Meanwhile, GEN also could be determined due to the cross reaction with DZ monoclonal antibody (MAb). Fluorescein isothiocyanate (FITC) was used to label the pure DZ antigen to compete with DZ in toy products. Magnetic beads (MBs) capped with anti-FITC antibody were both the solid phase and separator. The chemiluminescence signal produced by the emission of photons from ABEI was inversely proportional to the amount of DZ and GEN in the sample solution. The method was linear to 6000 ng/mL DZ with a detection limit of 1.34 ng/mL. The intra-assay imprecision results [mean (coefficient of variation, CV)] were less than 6%. The average recoveries ranged from 92 to 98%. The relationship between the proposed method and high-performance liquid chromatography (HPLC) exhibited a correlation. The proposed method demonstrates acceptable performance for rapid quantification of DZ and GEN and can be used to rapidly detect these substances in soy products.

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Authors and Affiliations

  1. National-Regional Key Technology Engineering Laboratory for Medical Ultrasound, Guangdong Key Laboratory for Biomedical Measurements and Ultrasound Imaging, Department of Biomedical Engineering, School of Medicine, Shenzhen University, Shenzhen, 518060, China

    Suwen Qi, Depeng Xu & Qiwen Chen

  2. Department of Laboratory Medicine, The Second Hospital Affiliated to Chongqing Medical University, Linjiang Road 74, Chongqing, 400016, People’s Republic of China

    Pu Li

  3. Medical School, Shenzhen Second Hospital (The First Affiliated Hospital of Shenzhen University), Nanhai Ave 3688, Shenzhen, Guangdong Province, 518060, People’s Republic of China

    Ni Xie & Qin Huo

Authors
  1. Suwen Qi
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  2. Depeng Xu
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  3. Qiwen Chen
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  4. Pu Li
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  5. Ni Xie
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  6. Qin Huo
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Correspondence to Pu Li or Ni Xie.

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Funding

This work was supported by the Project of the National Science Foundation of China (Grant nos. 61401285 and 81401750), Guangdong Science Plan (Nos. 2013B021800097 and 2014A020212038), and Shenzhen Science Plan (Nos. JCYJ20160307114925241 JCYJ20140418182819179, JCYJ20150330102720122, and cxzz201418182638764).

Conflict of Interest

Suwen Qi declares that she has no conflict of interest. Depeng Xu declares that he has no conflict of interest. Qiwen Chen declares that he has no conflict of interest. Pu Li declares that he has no conflict of interest. Ni Xie declares that he has no conflict of interest. Qin Huo declares that he has no conflict of interest.

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This article does not contain any studies with human or animal subjects.

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Qi, S., Xu, D., Chen, Q. et al. Indirect Competitive Chemiluminescence Immunoassay for Glycosides Daidzin and Genistin in Soy Products. Food Anal. Methods 11, 367–373 (2018). https://doi.org/10.1007/s12161-017-1007-y

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  • DOI: https://doi.org/10.1007/s12161-017-1007-y

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