Abstract
As a synthetic food dye, erythrosine is associated with serious toxicity in inducing thyroid tumors, and the use of erythrosine is strictly regulated in most counties including China. In this study, a direct enzyme-linked immunosorbent assay (ELISA) has been developed for analysis of erythrosine in food products. A highly specific monoclonal antibody (MAb) for erythrosine was produced using erythrosine–bovine serum albumin (BSA) conjugate as an artificial antigen, and horseradish peroxidase (HRP)-labeled MAb for erythrosine was utilized as the detection antibody. Under the optimized condition, the UV absorbance in microplate related well with erythrosine concentration in the range of 0.1–10.0 μg/g. The proposed method could be applied to determine erythrosine in beverage and cookie, with good recoveries (80 –103 %) for the three spiked levels (30, 50, and 100 μg/g), and the relative standard deviations of detected amount were <12.3 %.
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Acknowledgments
This work was supported by the financial support of National Natural Science Foundation (nos. 81173017 and 31101277), Tianjin Science and Technology Program (nos. 11ZCGHHZ01200, 12ZXCXSY08400, and 12JCQNJC08900), Project of Tianjin Innovation Found for Technology Based Firms (no. 11ZXCXSY04500), the Ministry of Science and Technology of the People’s Republic of China (2011YQ030134), and the Fundamental Research Funds for the Central Universities (no. 65011751).
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All authors declare that they have no conflict of interest.
Ethical Standards—Informed Consent
This article does not contain studies with human subjects.
Ethical Standards—Animal Rights
The studies with animals were conducted in compliance with the institutional and national guidelines for care and use of laboratory animals. This is also indicated in the “Preparation of Monoclonal Antibody for Erythrosine” section.
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Bo Zhang and Daolin Du The first two authors contributed equally to this work.
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Zhang, B., Du, D., Yin, Y. et al. A Direct Enzyme Immunoassay to Detect Erythrosine in Foods. Food Anal. Methods 7, 1798–1803 (2014). https://doi.org/10.1007/s12161-014-9824-8
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DOI: https://doi.org/10.1007/s12161-014-9824-8