Abstract
Multinucleated giant cell (MGC) reaction in oral squamous cell carcinoma (OSCC) usually represents a stromal foreign body reaction to keratin from neoplastic epithelial cells. We describe and illustrate by double immunohistochemistry a case of a tongue squamous cell carcinoma (SCC) in a 70-year-old female patient, with a copious MGC reaction not associated to keratin, showing a histopathological pattern not described before. The MGCs were directly associated with neoplastic cells, which are phagocytosed by the MGCs. Immunohistochemistry for CD68, AE1/AE3, CD163, CD11c, RANK, RANK-L, OPG were performed, as well as double staining for CD68 and AE1/AE3 to better illustrate the relationship between MGCs and neoplastic cells. The clinical and biological significance of this pattern of MGC reaction in OSCC needs to be better elucidated.
Similar content being viewed by others
References
De Wever O, Mareel M. Role of tissue stroma in cancer cell invasion. J Pathol. 2003;200(4):429–47.
Visser KE, Coussens LM. The inflammatory tumor microenvironment and its impact on cancer development. Contrib Microbiol. 2006;13:118–37.
Essa AA, Yamazaki M, Maruyama S, Abé T, Babkair H, Raghib AM, et al. Tumour-associated macrophages are recruited and differentiated in the neoplastic stroma of oral squamous cell carcinoma. Pathology 2016;48(3):219–27.
Ma J, Liu L, Che G, Yu N, Dai F, You Z. The M1 form of tumor-associated macrophages in non-small cell lung cancer is positively associated with survival time. BMC Cancer 2010;10:112.
Laoui D, Movahedi K, Van Overmeire E, Van den Bossche J, Schouppe E, et al. Tumor-associated macrophages in breast cancer: distinct subsets, distinct functions. Int J Dev Biol. 2011;55(7–9):861–7.
Falleni M, Savi F, Tosi D, Agape E, Cerri A, Moneghini L, et al. M1 and M2 macrophages’ clinicopathological significance in cutaneous melanoma. Melanoma Res 2017;27(3):200–10.
Hu JM, Liu K, Liu JH, Jiang XL, Wang XL, Chen YZ, et al. CD163 as a marker of M2 macrophage, contribute to predicte aggressiveness and prognosis of Kazakh esophageal squamous cell carcinoma. Oncotarget 2017;8(13):21526–38.
Burkhardt A, Höltje WJ. The effects of intra-arterial bleomycin therapy on squamous cell carcinoma of the oral cavity. Biopsy and autopsy examinations. J Maxillofac Surg. 1975;3(4):217–30.
Helming L, Gordon S. The molecular basis of macrophage fusion. Immunobiology. 2007;212(9–10):785–93.
Brooks E, Simmons-Arnold L, Naud S, Evans MF, Elhosseiny A. Multinucleated giant cells’ incidence, immune markers, and significance: a study of 172 cases of papillary thyroid carcinoma. Head Neck Pathol. 2009;3:95–9.
Patil S, Rao RS, Ganavi BS. A foreigner in squamous cell carcinoma! J Int Oral Health. 2013;5(5):147–50.
Santos HB, Miguel MC, Pinto LP, Gordón-Núñez MA, Alves PM, Nonaka CF. Multinucleated giant cell reaction in lower lip squamous cell carcinoma: a clinical, morphological, and immunohistochemical study. J Oral Pathol Med. 2017;46:773–9.
Reid MD, Muraki T, HooKim K, Memis B, Graham RP, Allende D, et al. Cytologic features and clinical implications of undifferentiated carcinoma with osteoclastic giant cells of the pancreas: an analysis of 15 cases. Cancer. 2017;125:563–75.
Weber M, Iliopoulos C, Moebius P, Büttner-Herold M, Amann K, Ries J, Preidl R, et al. Prognostic significance of macrophage polarization in early stage oral squamous cell carcinomas. Oral Oncol. 2016;52:75–84.
Petruzzi MN, Cherubini K, Salum FG, de Figueiredo MA. Role of tumour-associated macrophages in oral squamous cells carcinoma progression: an update on current knowledge. Diagn Pathol. 2017;12(1):32.
Brandwein-Gensler M, Teixeira MS, Lewis CM, Lee B, Rolnitzky L, Hille JJ, et al. Oral squamous cell carcinoma: histologic risk assessment, but not margin status, is strongly predictive of local disease-free and overall survival. Am J Surg Pathol. 2005;29(2):167–78.
Essa AA, Yamazaki M, Maruyama S, Abé T, Babkair H, Cheng J, et al. Keratin pearl degradation in oral squamous cell carcinoma: reciprocal roles of neutrophils and macrophages. J Oral Pathol Med. 2014;43(10):778–84.
Emanuel PO, Shim H, Phelps RG. Poorly differentiated squamous cell carcinoma with osteoclastic giant-cell-like proliferation. J Cutan Pathol. 2007;34:930–3.
Sica A, Larghi P, Mancino A, Rubino L, Porta C, Totaro MG, et al. Macrophage polarization in tumour progression. Semin Cancer Biol. 2008;18(5):349–55.
Won KY, Kalil RK, Kim YW, Park YK. RANK signalling in bone lesions with osteoclast-like giant cells. Pathology. 2011;43(4):318–21.
Taylor RM, Kashima TG, Hemingway FK, Dongre A, Knowles HJ, Athanasou NA. CD14- mononuclear stromal cells support (CD14+) monocyte-osteoclast differentiation in aneurysmal bone cyst. Lab Invest. 2012;92(4):600–5.
Branstetter D, Rohrbach K, Huang LY, Soriano R, Tometsko M, Blake M, et al. RANK and RANK ligand expression in primary human osteosarcoma. J Bone Oncol. 2015;4(3):59–68.
Khan UA, Hashimi SM, Khan S, Quan J, Bakr MM, Forwood MR, et al. Differential expression of chemokines, chemokine receptors and proteinases by foreign body giant cells (FBGCs) and osteoclasts. J Cell Biochem. 2014;115(7):1290–8.
Chuang FH, Hsue SS, Wu CW, Chen YK. Immunohistochemical expression of RANKL, RANK, and OPG in human oral squamous cell carcinoma. J Oral Pathol Med. 2009;38(10):753–8.
Zhang X, Junior CR, Liu M, Li F, D’Silva NJ, Kirkwood KL. Oral squamous carcinoma cells secrete RANKL directly supporting osteolytic bone loss. Oral Oncol. 2013;49(2):119–28.
Author information
Authors and Affiliations
Corresponding author
Ethics declarations
Conflict of interest
All of authors have indicated they have no potential conflicts of interest and no financial relationships relevant to this article to disclose.
Ethical Approval
This article does not contain any studies with animals performed by any of the authors. All procedures performed in this study involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards.
Informed Consent
Informed consent was obtained from all individual participants included in the study.
Rights and permissions
About this article
Cite this article
Sánchez-Romero, C., Carlos, R., Dantas Soares, C. et al. Unusual Multinucleated Giant Cell Reaction in a Tongue Squamous Cell Carcinoma: Histopathological and Immunohistochemical Features. Head and Neck Pathol 12, 580–586 (2018). https://doi.org/10.1007/s12105-018-0892-8
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s12105-018-0892-8