Abstract
Aberrant NSD2 methyltransferase activity is implicated as the oncogenic driver in multiple myeloma, suggesting opportunities for novel therapeutic intervention. The methyltransferase activity of NSD2 resides in its catalytic SET domain, which is conserved among most lysine methyltransferases. Here we report the backbone \(\hbox {H}^{\mathrm{N}}\), N, C\(^{\prime }\), \(\hbox {C}^\alpha\) and side-chain \(\hbox {C}^\beta\) assignments of a 25 kDa NSD2 SET domain construct, spanning residues 991–1203. A chemical shift analysis of C\(^{\prime }\), \(\hbox {C}^\alpha\) and \(\hbox {C}^\beta\) resonances predicts a secondary structural pattern that is in agreement with homology models.
![](http://media.springernature.com/m312/springer-static/image/art%3A10.1007%2Fs12104-016-9689-4/MediaObjects/12104_2016_9689_Fig1_HTML.gif)
![](http://media.springernature.com/m312/springer-static/image/art%3A10.1007%2Fs12104-016-9689-4/MediaObjects/12104_2016_9689_Fig2_HTML.gif)
![](http://media.springernature.com/m312/springer-static/image/art%3A10.1007%2Fs12104-016-9689-4/MediaObjects/12104_2016_9689_Fig3_HTML.gif)
Similar content being viewed by others
References
Delaglio F, Grzesiek S, Vuister GW, Zhu G, Pfeifer J, Bax A (1995) NMRPipe: a multidimensional spectral processing system based on UNIX pipes. J Biomol NMR 6(3):277–293
Grzesiek S, Bax A (1992) An efficient experiment for sequential backbone assignment of medium-sized isotopically enriched proteins. J Magn Reson 99:201–207
Hamamoto R, Saloura V, Nakamura Y (2015) Critical roles of non-histone protein lysine methylation in human tumorigenesis. Nat Rev Cancer 15(2):110–124
Helmus JJ, Jaroniec CP (2013) Nmrglue: an open source Python package for the analysis of multidimensional NMR data. J Biomol NMR 55(4):355–367
Hyberts SG, Milbradt AG, Wagner AB, Arthanari H, Wagner G (2012) Application of iterative soft thresholding for fast reconstruction of NMR data non-uniformly sampled with multidimensional Poisson Gap scheduling. J Biomol NMR 52(4):315–327
Kay LE, Ikura M, Tschudin R, Bax A (1990) Three-dimensional triple-resonance NMR spectroscopy of isotopically enriched proteins. J Magn Reson 89:496–514
MacRaild CA, Norton RS (2014) RASP: rapid and robust backbone chemical shift assignments from protein structure. J Biomol NMR 58(3):155–163
Markley JL, Bax A, Arata Y, Hilbers CW, Kaptein R, Sykes BD, Wright PE, Wüthrich K (1998) Recommendations for the presentation of NMR structures of proteins and nucleic acids. J Mol Biol 280(5):933–952
Qiao Q, Li Y, Chen Z, Wang M, Reinberg D, Xu RM (2011) The structure of NSD1 reveals an autoregulatory mechanism underlying histone H3K36 methylation. J Biol Chem 286(10):8361–8368
Rice JC, Nishioka K, Sarma K, Steward R, Reinberg D, Allis CD (2002) Mitotic-specific methylation of histone H4 Lys 20 follows increased PR-Set7 expression and its localization to mitotic chromosomes. Genes Dev 16(17):2225–2230
Shen Y, Delaglio F, Cornilescu G, Bax A (2009) TALOS+: a hybrid method for predicting protein backbone torsion angles from NMR chemical shifts. J Biomol NMR 44(4):213–223
Vranken WF, Boucher W, Stevens TJ, Fogh RH, Pajon A, Llinas M, Ulrich EL, Markley JL, Ionides J, Laue ED (2005) The CCPN data model for NMR spectroscopy: development of a software pipeline. Proteins 59(4):687–696
Wagner EJ, Carpenter PB (2012) Understanding the language of Lys36 methylation at histone H3. Nat Rev Mol Cell Biol 13(2):115–126
Walser R, Renshaw J, Milbradt AG (2016) Backbone resonance assignments for the PHD-Bromo dual-domain of the human chromatin reader TRIM24. Biomol NMR Assign 10(1):207–211
Acknowledgments
We would like to thank Dr. Paul Wan for discussions around protein expression and purification and Dr. Martin Packer for providing the homology model of NSD2 SET domain. We are grateful to Dr. Kevin Embrey for reading the manuscript prior to submission.
Author information
Authors and Affiliations
Corresponding author
Electronic supplementary material
Below is the link to the electronic supplementary material.
Rights and permissions
About this article
Cite this article
Bobby, R., Peciak, K. & Milbradt, A.G. Backbone resonance assignments for the SET domain of the human methyltransferase NSD2. Biomol NMR Assign 10, 307–310 (2016). https://doi.org/10.1007/s12104-016-9689-4
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s12104-016-9689-4