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Serotype Determination of Adenoviruses in Children with Respiratory Infection

  • Original Article
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Abstract

Objective

To determine adenovirus serotypes among children with acute respiratory infection (ARI) in Khoozestan province, Iran during years 2010–2011.

Methods

One hundred sixty three nasopharyngeal swabs were collected from children between 1 and 15 y who were hospitalized for the acute respiratory infection. The viral DNA was extracted from the nasopharyngeal swabs and adenoviruses were detected by Nested PCR. Positive PCR samples were sequenced in order to confirm the adenovirus serotypes.

Results

Out of 163 samples, 30 (18.4 %) were positive for adenoviruses by nested PCR. Twenty two PCR products were sequenced and recognized as Ad5 and Ad2 serotypes including 19 (86.3 %) Ad5 and 3 (13.7 %) Ad2.

Conclusions

This study reveals that adenoviruses with Ad5 predominance are important cause of respiratory tract infection in children.

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Acknowledgments

This study was done as part of thesis with 89108 registration number in Health research institute, Infectious and Tropical Diseases Research Center, Jundishapur University of Medical Science, Ahvaz, Iran. The authors are thankful to Director of Infectious and Tropical Disease Research Center, Ahwaz Jundishapur University of Medical Sciences, Ahwaz, Iran for the financial support to carry out this project.

Contributions

YG: Did experiments and manuscript preparation; MM: Study design, funding support and manuscript preparation; ARS: Manuscript preparation; AN, NN and SN: Did experiments; MR: Data analysis and manuscript preparation; KA: Statistical analysis; AS and RN: Clinical consultation. MM will act as guarantor for this paper.

Conflict of Interest

None.

Role of Funding Source

Infectious and Tropical Disease Research Center, Ahwaz Jundishapur University of Medical Sciences, Ahwaz, Iran.

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Authors and Affiliations

  1. Health Research Institute, Infectious and Tropical Disease Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran

    Manoochehr Makvandi, Ali Reza Samarbafzadeh & Ahmad Shamsizadeh

  2. Department of Virology, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran

    Yusuf Ghasemi, Manoochehr Makvandi, Ali Reza Samarbafzadeh, Ahmad Nejati, Saeed Najafifard, Niloofar Neisi & Mojtaba Rasti

  3. Department of Statistics, School of Health, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran

    Kambiz Ahmadi

  4. Pediatric Infectious Disease Section, Abozar Children’s Hospital, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran

    Ahmad Shamsizadeh & Roya Nikfar

  5. Golestan Boulevard, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran

    Manoochehr Makvandi

Authors
  1. Yusuf Ghasemi
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  2. Manoochehr Makvandi
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Correspondence to Manoochehr Makvandi.

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Ghasemi, Y., Makvandi, M., Samarbafzadeh, A.R. et al. Serotype Determination of Adenoviruses in Children with Respiratory Infection. Indian J Pediatr 81, 639–643 (2014). https://doi.org/10.1007/s12098-013-1286-2

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  • DOI: https://doi.org/10.1007/s12098-013-1286-2

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