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Lipid Production by Rhodotorula glutinis in Continuous Cultivation with a Gravity Sedimentation System

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Abstract

Lipid accumulation is generally believed to be a partially growth-coupled biochemical process that results in differences in lipid content between different cells. To separate lipid-rich cells and increase the cellular biomass in bioreactors during the cultivation of the oleaginous yeasts, a gravity sedimentation system (GSS) is coupled to a bioreactor. The dilution rate (D) and the ratio of the outflow rate from the outlet of the GSS to the inflow rate into the bioreactor (B) were evaluated. The biomass in the bioreactor with GSS increased by 16.3% and 30.6% at D values of 0.05 h−1 (B = 0.25) and 0.02 h−1 (B = 0.5), respectively. Interestingly, cells containing 39.3% lipids were obtained from the outlet of the GSS (D = 0.02 h−1, B = 0.5), and the lipid content increased by 7.8% compared to that of the bioreactor. The results indicated that use of a GSS is a very effective method for increasing the cell concentration and separation of lipid-rich cells.

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Acknowledgements

This research was supported the State Key Laboratory of Motor Vehicle Biofuel Technology (KFKT2014009).

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Authors and Affiliations

  1. Dalian Xinyulong Marine Biological Seed Industry Technology Company Limited, 4 Luxun Rd., Dalian, 116023, People’s Republic of China

    Hongwei Shen

  2. Division of Biotechnology, Dalian Institute of Chemical Physics, CAS, 457 Zhongshan Rd., Dalian, 116023, People’s Republic of China

    Hongwei Shen, Qiang Li & Xue Yu

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  1. Hongwei Shen
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Correspondence to Hongwei Shen.

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Shen, H., Li, Q. & Yu, X. Lipid Production by Rhodotorula glutinis in Continuous Cultivation with a Gravity Sedimentation System. Indian J Microbiol 60, 246–250 (2020). https://doi.org/10.1007/s12088-019-00849-3

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