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Isolation and Characterization of Chromium(VI)-Reducing Bacteria from Tannery Effluents

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Abstract

Two chromium-resistant bacteria (IFR-2 and IFR-3) capable of reducing/transforming Cr(VI) to Cr(III) were isolated from tannery effluents. Isolates IFR-2 and IFR-3 were identified as Staphylococcus aureus and Pediococcus pentosaceus respectively by 16S rRNA gene sequence analyses. Both isolates can grow well on 2,000 mg/l Cr(VI) (as K2Cr2O7) in Luria-Bertani (LB) medium. Reduction of Cr(VI) was found to be growth-associated in both isolates and IFR-2 and IFR-3 reduced 20 mg/l Cr(VI) completely in 6 and 24 h respectively. The Cr(VI) reduction due to chromate reductase activity was detected in the culture supernatant and cell lysate but not at all in the cell extract supernatant of both isolates. Whole cells of IFR-2 and IFR-3 converted 24 and 30% of the initial Cr(VI) concentration (1 mg/l) in 45 min respectively at 37°C. NiCl2 stimulated the growth of IFR-2 whereas HgCl2 and CdCl2 significantly inhibited the growth of both isolates. Optimum temperature and pH for growth of and Cr(VI) reduction by both isolates were found to be between 35 and 40°C and pH 7.0 to 8.0. The two bacterial isolates can be good candidates for detoxification of Cr(VI) in industrial effluents.

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References

  1. McLean J, Beveridge TJ (2001) Chromate reduction in Pseudomonad isolated from a site contaminated with chromate copper arsenate. Appl Environ Microbiol 67:1076–1084

    Article  PubMed  CAS  Google Scholar 

  2. Rai D, Sass BM, Moore DA (1987) Chromium(III) hydrolysis constants and solubility of chromium(III) hydroxide. Inorg Chem 26:345–349

    Article  CAS  Google Scholar 

  3. Yang J, Minyan H, Wang G (2009) Removal of toxic chromate using free and immobilized Cr(VI)-reducing bacterial cells of Intrasporangium sp. Q5-1. World J Microbiol Biotechnol 25:1579–1587

    Article  CAS  Google Scholar 

  4. Camargo FAO, Bento FM, Okeke BC, Frankenberger WT (2003) Chromate reduction by chromium-resistant bacteria isolated from soils contaminated with dichromate. J Environ Qual 32:1228–1233

    Article  PubMed  CAS  Google Scholar 

  5. Shen H, Yi-Tin W (1993) Characterization of enzymatic reduction of hexavalent chromium by Escherichia coli ATCC 33456. Appl Environ Microbiol 59(11):3771–3777

    PubMed  CAS  Google Scholar 

  6. Wang PC, Mori T, Komori K, Sasatsu M, Toda K, Ohtake H (1989) Isolation and characterization of Enterobacter cloacae strain that reduces hexavalent chromium under anaerobic conditions. Appl Environ Microbiol 55:1665–1669

    PubMed  CAS  Google Scholar 

  7. Bopp LH, Chakrabarty AM, Ehrlich HL (1983) Chromate resistance plasmid in Pseudominas fluorescens. J Bacteriol 155:1105–1109

    PubMed  CAS  Google Scholar 

  8. Ganguli A, Tripathi AK (2002) Bioremediation of toxic chromium from electroplating effluent by chromate reducing Pseudomonas aeruginosa A2Chr in two bioreactors. Appl Microbiol Biotechnol 58:416–420

    Article  PubMed  CAS  Google Scholar 

  9. Sambrook J, Fritsch EF, Miniatis T (1989) Molecular cloning: a laboratory manual, 2nd edn. Cold Spring Harbor Laboratory Press, Cold Spring Harbor

    Google Scholar 

  10. Ovreås L, Forney L, Daae FL, Torsvik V (1997) Distribution of bacterioplankton in meromictic lake Saelenvannet, as determined by denaturing gradient gel electrophoresis of PCR amplified gene fragments coding for 16S rRNA. Appl Environ Microbiol 63:3367–3373

    PubMed  Google Scholar 

  11. Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ (1990) Basic local alignment search tool. J Mol Biol 215:403–410

    PubMed  CAS  Google Scholar 

  12. Bartlett RJ, James BR (1996) Chromium. In: Sparks DL (ed) Methods of soil analysis, Part 3, SSSA Book Series 5. SSSA, Madison, pp 683–701

    Google Scholar 

  13. Camargo FAO, Okeke BC, Bento FM, Frankenberger WT (2003) In vitro reduction of hexavalent chromium by a cell-free extract of Bacillus sp. ES29 stimulated by Cu2+. Appl Microbiol Biotechnol 62:569–573

    Article  PubMed  CAS  Google Scholar 

  14. Thacker U, Madamwar D (2005) Reduction of toxic chromium and partial localization of chromium reductase activity in bacterial isolate DM1. World J Microbiol Biotechnol 21:891–899

    Article  CAS  Google Scholar 

  15. Wang PC, Mori T, Toda K, Ohtake H (1990) Membrane associated chromate reductase activity from Enterobacter cloacae. J Bacteriol 172:1670–1672

    PubMed  CAS  Google Scholar 

  16. McLean SJ, Beveridge TJ, Phipps D (2000) Isolation and characterization of a chromium-reducing bacterium from a chromated copper arsenate-contaminated site. Environ Microbiol 2(6):611–619

    Article  PubMed  CAS  Google Scholar 

  17. Basu M, Bhattacharya S, Paul AK (1997) Isolation and characterization of chromium-resistant bacteria from tannery effluents. Bull Environ Contam Toxicol 58:535–542

    Article  PubMed  CAS  Google Scholar 

  18. Suzuki T, Miyata N, Horitsu H, Kawai K, Takamizawa K, Yutaka Tai Y, Okazaki M (1992) NAD(P)H-dependent chromium(VI) reductase of Pseudomonas ambiga G-1.: a Cr(V) intermediate formed during the reduction of Cr(VI) to Cr(III). J Bacteriol 174:5340–5345

    PubMed  CAS  Google Scholar 

  19. Shuttleworth KL, Richard FU (1993) Sorption of heavy metals to the filamentous bacterium Thiothrix strain Al. Appl Environ Microbiol 59:1274–1282

    PubMed  CAS  Google Scholar 

  20. Lovely DR, Philips EJP (1994) Reduction of chromate by Desulfovibrio vulgaris and its C3 cytochrome. Appl Environ Microbiol 60:726–728

    Google Scholar 

  21. Ramírez-Díaz MI, Díaz-Pérez C, Vargas E, Riveros-Rosas H, Campos-García J, Cervantes C (2008) Mechanisms of bacterial resistance to chromium compounds. Biometals 21:321–332

    Article  PubMed  Google Scholar 

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Acknowledgment

This work was supported with grant in aid by the Ministry of Science and Information and Communication Technology, Government of the People’s Republic of Bangladesh.

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Authors and Affiliations

  1. Department of Microbiology, University of Dhaka, Dhaka, 1000, Bangladesh

    Mohammad Ilias, Iftekhar Md. Rafiqullah, Bejoy Chandra Debnath, Khanjada Shahnewaj Bin Mannan & Md. Mozammel Hoq

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  1. Mohammad Ilias
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  2. Iftekhar Md. Rafiqullah
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  3. Bejoy Chandra Debnath
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  4. Khanjada Shahnewaj Bin Mannan
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  5. Md. Mozammel Hoq
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Correspondence to Md. Mozammel Hoq.

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Ilias, M., Rafiqullah, I.M., Debnath, B.C. et al. Isolation and Characterization of Chromium(VI)-Reducing Bacteria from Tannery Effluents. Indian J Microbiol 51, 76–81 (2011). https://doi.org/10.1007/s12088-011-0095-4

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  • DOI: https://doi.org/10.1007/s12088-011-0095-4

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