Abstract
Chromium-resistant bacteria (CRB) isolated from soils can be used to reduce toxic Cr(VI) from contaminated environments. This study assessed in vitro reduction of hexavalent Cr using a cell-free extract (CFE) of CRB isolated from soil contaminated with dichromate. One isolate, ES 29, that substantially reduced Cr(VI) was identified as a Bacillus species by 16S rRNA gene-sequence homology. The isolate reduced Cr(VI) under aerobic conditions, using NADH as an electron donor and produced a soluble Cr(VI)-reducing enzyme stimulated by copper (Cu2+). The CFE of the bacterial isolate reduced 50% of Cr(VI) in 6 h. The Cr(VI)-reduction activity of the CFE had a K m of 7.09 μM and a V max of 0.171 μmol min−1 mg−1 protein. Mercury inhibited the enzyme, but not competitively, with a V max of 0.143 μmol min−1 mg−1 protein, a K m of 7.07 μM and a K i of 1.58 μM. This study characterizes the enzymatic reduction of Cr(VI) by Bacillus sp. ES 29 which can be used for the bioremediation of chromate.
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Acknowledgements
>F.A.O. Camargo and F.M. Bento are grateful to the Brazilian National Research Council (CNPq) for a scholarship concession, especially to SEBIE staff Jose Airton de Souza and to the Department of Environmental Science (UCR) for the opportunity to participate as post-doctoral scientists. The authors declare that the experiments comply with the current laws of the country in which the experiment was performed.
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Camargo, F.A.O., Okeke, B.C., Bento, F.M. et al. In vitro reduction of hexavalent chromium by a cell-free extract of Bacillus sp. ES 29 stimulated by Cu2+ . Appl Microbiol Biotechnol 62, 569–573 (2003). https://doi.org/10.1007/s00253-003-1291-x
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DOI: https://doi.org/10.1007/s00253-003-1291-x