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Dietary intake of Spirulina platensis alters HSP70 gene expression profiles in the brain of rats in an experimental model of mixed stress

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Abstract

Spirulina platensis has gradually gained more attention for its therapeutic, antioxidant, and anti-inflammatory potential worldwide. However, the current molecular knowledge about the effects of spirulina on stress-related genes is rather limited. The effects of dietary intake of spirulina on the HSP70 gene expression were assessed in a controlled in vivo experimental design. Moreover, alterations in serum corticosterone levels, IL-2, IL-4, IFN-γ, triglyceride, ALT, AST, relative gene expression values, and the correlations between them were evaluated. A total of 36 rats were divided into four groups: control group, stress-only group, spirulina group, and spirulina+stress group. To control the dose administration, S. platensis was applied by a gastric gavage in stress groups. Crowded environment stress and hosting alone stress were applied to the stress-only group and spirulina + stress group. RNA was extracted from brain samples using TRIpure and the relative gene expression assessment was performed using Roche-LightCycler-480-II real-time PCR-System. Gene expression values were remarkably different among the four experimental groups. The differences between stress-only and the spirulina groups were statistically significant (P<0.05). The correlation between the HSP70 gene expression and the IFN-γ was found to be statistically significant (P<0.05; r=0.50). Results indicate a novel effect of spirulina on the HSP70 expression related to the stress-response. Data presented in this study may be useful for further studies to define not only the molecular genetic aspects through dietary S. platensis but also the effects of spirulina on stress-response and animal welfare.

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Acknowledgements

The data on some parts of the research has been presented as an oral presentation at the 2nd International Eurasian Conference on Science, Engineering and Technology (EurasianSciEnTech 2020), Gaziantep, Turkey. Eda Koseli was supported by YÖK (Council of Higher Education) 100/200 Doctoral Research Fellowship Programme and TÜBİTAK (The Scientific and Technological Research Council of Turkey) 2211-C (National Ph.D. Scholarship Programme in the Priority Fields in Science and Technology). The authors are grateful to Dr Ozge Ardicli for her support.

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  1. Faculty of Veterinary Medicine, Department of Genetics, Bursa Uludag University, Bursa, 16059, Turkey

    SENA ARDICLI

  2. Faculty of Veterinary Medicine, Department of Physiology, Tekirdag Namik Kemal University, Tekirdag, 59030, Turkey

    NILAY SEYIDOGLU

  3. Faculty of Veterinary Medicine, Department of Physiology, Bursa Uludag University, Bursa, 16059, Turkey

    EDA KOSELI, ROVSHAN GURBANLI & CENK AYDIN

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  1. SENA ARDICLI
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ARDICLI, S., SEYIDOGLU, N., KOSELI, E. et al. Dietary intake of Spirulina platensis alters HSP70 gene expression profiles in the brain of rats in an experimental model of mixed stress. J Genet 101, 49 (2022). https://doi.org/10.1007/s12041-022-01388-5

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