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Molecular mapping of CLCuD resistance introgressed from synthetic cotton polyploid in upland cotton

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Abstract

Cotton leaf curl disease (CLCuD), caused by a geminivirus complex, is the most serious disease of upland cotton in northwest India and Pakistan. It results in substantial losses in cotton yield and fibre quality. Due to continuous appearance of new viral strains, all the established CLCuD resistant stocks, extant and obsolete cultivars of upland cotton have become susceptible. Therefore, it became crucial to explore the novel sources of CLCuD resistance, as development of CLCuD resistant varieties is the most practical approach to manage this menace. Here, for the first time, we report introgression and mapping of CLCuD resistance from a ‘synthetic cotton polyploid’ to upland cotton. A backcross population (synthetic polyploid / Gossypium hirsutum Acc. PIL 43/G. hirsutum Acc. PIL 43) was developed for studying inheritance and mapping of CLCuD resistance. Dominance of CLCuD resistance was observed over its susceptibility. Two dominant genes were found to confer resistance to CLCuD. Molecular analysis through genotyping-by-sequencing revealed that chromosomes A01 and D07 harboured one CLCuD resistance gene each.

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Acknowledgements

The research work was carried out under the Programme Support on ‘Enhancing Durability of Resistance to Biotic Stresses in Selected Cereal and Fibre Crops through Biotechnological Approaches (BT/01/CE1B/121/01)’ funded by the Department of Biotechnology, Government of India. Ministry of Science and Technology (Grant No. 102/IFD/SAN/1307/2014-15).

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Authors and Affiliations

  1. Department of Plant Breeding and Genetics, Punjab Agricultural University, Ludhiana, 141 004, India

    Suruchi Vij, Dharminder Pathak & Dharminder Bhatia

  2. PAU Regional Research Station, Faridkot, 151 203, India

    Pankaj Rathore & Harish Kumar

  3. Department of Plant Pathology, Punjab Agricultural University, Ludhiana, 141 004, India

    P. S. Sekhon

  4. School of Agricultural Biotechnology, Punjab Agricultural University, Ludhiana, 141 004, India

    Parveen Chhuneja

  5. ICAR-National Bureau of Plant Genetic Resources, New Delhi, 110 012, India

    Kuldeep Singh

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  1. Suruchi Vij
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  2. Dharminder Pathak
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Correspondence to Dharminder Pathak.

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Corresponding editor: Manoj Prasad

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Vij, S., Pathak, D., Rathore, P. et al. Molecular mapping of CLCuD resistance introgressed from synthetic cotton polyploid in upland cotton. J Genet 101, 25 (2022). https://doi.org/10.1007/s12041-022-01365-y

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