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Dominance of resistance-breaking cotton leaf curl Burewala virus (CLCuBuV) in northwestern India

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Abstract

Cotton leaf curl disease (CLCuD) is a major limitation to cotton production on the Indian subcontinent. A survey for viruses causing CLCuD was conducted during the 2009 and 2010 cropping seasons in the northwestern Indian cotton-growing belt in the states of Punjab, Haryana and Rajasthan. Partial sequences of 258 and full-length sequences of 22 virus genomes were determined. This study shows that the resistance-breaking cotton leaf curl Burewala virus (CLCuBuV) is now the dominant virus in many fields. The spread and establishment of the mutant CLCuBuV in northwestern India, the variation in its genomic sequence, its virulence and infectivity, and the implications for cotton breeding are discussed.

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Acknowledgments

This research was partially funded by an ‘SBIRI’ grant (No.BT/04(SBIRI)/05/2006) from the Biotech Consortium India Limited, Department of Biotechnology, India, to Maharashtra Hybrid Seeds Company Limited (MHSCL), Maharashtra, India. We thank the cotton farmers for giving access to their fields and for providing information on prevalence of diseases during our field visits. We are thankful to Dr. A. K. Agarwal and his team for their assistance during the 2010 survey. We are very grateful to Dr. R.W. Briddon for discussions, valuable suggestions, and critical review of the manuscript. We would also like to thank Dr. H. S. Savithri and Dr. Bharat Char for their input.

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Authors and Affiliations

  1. Plant-Virus Interactions Lab, Mahyco Research Center, Maharashtra Hybrid Seeds Company Limited, Dawalwadi, Post Box no-76, Jalna, Maharashtra, 431 203, India

    Prem A. Rajagopalan, Amruta Naik, Prashanth Katturi, Meera Kurulekar & Radhamani Anandalakshmi

  2. Vegetable Research Center, Maharashtra Hybrid Seeds Company Limited, Bettanagera village, Huskur Post, Bangalore, 562 123, India

    Ravi S. Kankanallu

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  1. Prem A. Rajagopalan
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  2. Amruta Naik
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  3. Prashanth Katturi
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  5. Ravi S. Kankanallu
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  6. Radhamani Anandalakshmi
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Correspondence to Radhamani Anandalakshmi.

Additional information

The full-length nucleotide sequences of DNA A and DNA beta of 29 cotton leaf curl–associated begomoviruses (CBVs) were characterized in this study. The sequences were submitted to NCBI GenBank, and accession numbers are presented in Table 2.

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Rajagopalan, P.A., Naik, A., Katturi, P. et al. Dominance of resistance-breaking cotton leaf curl Burewala virus (CLCuBuV) in northwestern India. Arch Virol 157, 855–868 (2012). https://doi.org/10.1007/s00705-012-1225-y

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