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Genetic interaction and mapping studies on the leaflet development (lld) mutant in Pisum sativum

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Abstract

In Pisum sativum, the completely penetrant leaflet development (lld) mutation is known to sporadically abort pinnae suborgans in the unipinnate compound leaf. Here, the frequency and morphology of abortion was studied in each of the leaf suborgans in 36 genotypes and in presence of auxin and gibberellin, and their antagonists. Various lld genotypes were constructed by multifariously recombining lld with a coch homeotic stipule mutation and with af, ins, mare, mfp, tl and uni-tac leaf morphology mutations. It was observed that the suborgans at all levels of pinna subdivisions underwent lld-led abortion events at different stages of development. As in leafblades, lld aborted the pinnae in leaf-like compound coch stipules. The lld mutation interacted with mfp synergistically and with other leaf mutations additively. The rod-shaped and trumpet-shaped aborted pea leaf suborgans mimicked the phenotype of aborted leaves in HD-ZIP-III-deficient Arabidopsis thaliana mutants. Suborganwise aborted morphologies in lld gnotypes were in agreement with basipetal differentiation of leaflets and acropetal differentiation in tendrils. Altogether, the observations suggested that LLD was the master regulator of pinna development. On the basis of molecular markers found linked to lld, its locus was positioned on the linkage group III of the P. sativum genetic map.

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Acknowledgements

The financial support of Indian National Science Academy and Council of Scientific and Industrial Research (CSIR) in the form of Scientistships to SK, and facilities provided by NIPGR are gratefully acknowledged. RKM, SC and AK, RK and VS respectively received postgraduate fellowship support from CSIR, Department of Biotechnology, and SKA Institution for Research, Education and Development. The technical help of Sanjay Kumar Rai, Vinod Kumar and Sunil Kumar is also thankfully acknowledged.

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  1. Genetical Genomics Laboratory, National Institute of Plant Genome Research (NIPGR), Aruna Asaf Ali Marg, New Delhi, 110 067, India

    SUSHIL KUMAR, RAGHVENDRA KUMAR MISHRA, ARVIND KUMAR, SWATI CHAUDHARY, VISHAKHA SHARMA & RENU KUMARI

  2. SKA Institution for Research, Education and Development (SKAIRED), 4/11 Sarv Priya Vihar, New Delhi, 110 016, India

    SUSHIL KUMAR, ARVIND KUMAR, SWATI CHAUDHARY, VISHAKHA SHARMA & RENU KUMARI

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[Kumar S., Mishra R. K., Kumar A., Chaudhary S., Sharma V. and Kumari R. 2012 Genetic interaction and mapping studies on the leaflet development (lld) mutant in Pisum sativum. J. Genet. 91, xx–xx]

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KUMAR, S., MISHRA, R.K., KUMAR, A. et al. Genetic interaction and mapping studies on the leaflet development (lld) mutant in Pisum sativum . J Genet 91, 325–342 (2012). https://doi.org/10.1007/s12041-012-0197-8

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