Skip to main content
SpringerLink
Log in

Construction of genetic linkage map of the medicinal and ornamental plant Catharanthus roseus

  • Research Article
  • Published:
Journal of Genetics Aims and scope Submit manuscript

Abstract

An integrated genetic linkage map of the medicinal and ornamental plant Catharanthus roseus, based on different types of molecular and morphological markers was constructed, using a F2 population of 144 plants. The map defines 14 linkage groups (LGs) and consists of 131 marker loci, including 125 molecular DNA markers (76 RAPD, 3 RAPD combinations; 7 ISSR; 2 EST-SSR from Medicago truncatula and 37 other PCR based DNA markers), selected from a total of 472 primers or primer pairs, and six morphological markers (stem pigmentation, leaf lamina pigmentation and shape, leaf petiole and pod size, and petal colour). The total map length is 1131.9 cM (centiMorgans), giving an average map length and distance between two markers equal to 80.9 cM and 8.6 cM, respectively. The morphological markers/genes were found linked with nearest molecular or morphological markers at distances varying from 0.7 to 11.4 cM. Linkage was observed between the morphological markers concerned with lamina shape and petiole size of leaf on LG1 and leaf, stem and petiole pigmentation and pod size on LG8. This is the first genetic linkage map of C. roseus.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Ammiraju J. S. S., Dholakia B. B., Santra D. K., Singh H., Tamhankar S. A., Dhaliwal H. S. et al. 2001 Identification of simple sequence repeat (ISSR) markers associated with seed size in wheat. Theor. Appl. Genet. 102, 726–732.

    Article  CAS  Google Scholar 

  • Becher R. 2007 EST-derived microsatellites as a rich source of molecular markers for oats. Plant Breeding DOI: 10.1111/j.1439-0523.2007.010330.x.

  • Debnata M., Malik C. P. and Bireu P. S. 2006 Micropropagation a tool for the production of high quantity plant. Curr. Pharm. Biotech. 7, 35–39.

    Google Scholar 

  • Facchini P. J. 2001 Alkaloid biosynthesis in plants: biochemistry, cell biology, molecular regulation and metabolic engineering applications. Annu. Rev. Plant Physiol. Plant Mol. Biol. 52, 29–66.

    Article  PubMed  CAS  Google Scholar 

  • Facchini P. J. and St-Pierre B. 2005 Synthesis and trafficking of alkaloid biosynthetic enzymes. Curr. Opin. Plant Biol. 6, 657–666.

    Article  Google Scholar 

  • Geerlings A., Redondo F. J., Contin A., Memelink J., van der Heijden R. and Verpoorte R. 2001 Biotransformation of tryptamine and secologanin into plant terpenoid indole alkaloids by transgenic yeast. Appl. Microbiol. Biotechnol. 56, 420–424.

    Article  PubMed  CAS  Google Scholar 

  • Gupta P. K. and Varshney R. K. 2000 The development and use of microsatellite markers for genetic analysis and plant breeding with emphasis on bread wheat. Euphytica 113, 163–185.

    Article  CAS  Google Scholar 

  • Jacobs D. I., Gaspari., van der Greef J., van der Heijden R. and Verpoorte R. 2005 Proteome analysis of the medicinal plant Catharanthus roseus. Planta 221, 690–704.

    Article  PubMed  CAS  Google Scholar 

  • Kojima T., Nagaoka T., Noda K. and Ogihara Y. 1998 Genetic linkage map of ISSR and RAPD markers in Einkorn wheat in relation to that of RFLP markers. Theor. Appl. Genet. 96, 37–45.

    Article  CAS  Google Scholar 

  • Kosambi D. D. 1944 The estimation of map distance from recombination values. Ann. Eugen. 12, 172–175.

    Google Scholar 

  • Kulkarni R. N., Baskaran K. and Sreevalli K. 2005 Genetics of novel corolla colours in periwinkle. Euphytica 144, 101–107.

    Article  Google Scholar 

  • Kulkarni R. N., Baskaran K., Chandrashekhara R. S. and Kumar S. 1999 Inheritance of morphological traits of periwinkle mutants with modified contents and yields of leaf and root alkaloids. Plant Breeding 118, 71–74.

    Article  CAS  Google Scholar 

  • Laucou V., Haurogne K., Ellis N. and Rameau C. 1998 Genetic mapping in pea. 1. RAPD-based genetic linkage map of Pisum sativum. Theor. Appl. Genet. 97, 905–915

    Article  CAS  Google Scholar 

  • Ledue M., Tikhomiroff C., Cloutier M., Perrier M. and Jolicoeur M. 2006 Development of a kinetic metabolic model: application to Catharanthus roseus hairy root. Biopro. Biosystem. Eng. 28, 295–313.

    Article  Google Scholar 

  • Leveque D., Wihlm J. and Jehl F. 1996 Pharmacology of Catharanthus alkaloids. Bull. Cancer 83, 176–186.

    PubMed  CAS  Google Scholar 

  • Loridon K., McPhee K., Morin J., Dubreuil P., Pilet-Nayel M. L., Aubert G. et al. 2005 Microsatellite marker polymorphism and mapping in pea (Pisum sativum L.). Theor. Appl. Genet. 111, 1022–1031.

    Article  PubMed  CAS  Google Scholar 

  • Lörz H. and Wenzel G. 2005 Molecular Marker Systems in Plant Breeding and Crop Improvement. Springer, Berlin.

    Book  Google Scholar 

  • Mahroug S., Courdavault V., Thiersault M., St-Pierre B. and Burlat V. 2006 Epidermis is a pivotal site of at least four secondary metabolic pathways in Catharanthus roseus aerial organs. Planta 223, 1191–2000.

    Article  PubMed  CAS  Google Scholar 

  • Manly K. F., Cudmore Jr. R. H. and Meer J. M. 2001 Map Manager QTX, cross-platform software for genetic mapping. Mammal. Genome 12, 930–932.

    Article  CAS  Google Scholar 

  • Meksem K. and Kahl G. 2005 The handbook of plant genome mapping: genetic and physical mapping. Wiley-VCH Verlag GmbH, Weinheim.

    Google Scholar 

  • Memelink J. 2005 The use of genetics to dissect plant secondary pathways. Curr. Opin. Plant Biol. 8, 230–235.

    Article  PubMed  CAS  Google Scholar 

  • Mishra P. and Kumar S. 2000 Emergence of periwinkle Catharanthus roseus as a model system for molecular biology of alkaloids: phytochemistry, pharmacology, plant biology and in vitro and in vivo cultivation. J. Med. Aromat. Plant Sci. 22, 306–337.

    CAS  Google Scholar 

  • Pandey-Rai S. and Kumar S. 2000 Induced mutation to monocotyledony in periwinkle, Catharanthus roseus, and suppression of mutant phenotype by kinetin. J. Genet. 79, 1–18.

    Article  Google Scholar 

  • Pandey-Rai S. and Kumar S. 2001 Heterocarpous flowers resulting from a recessive mutation in periwinkle, Catharanthus roseus. Curr. Sci. 80, 1581–1584.

    Google Scholar 

  • Pandey-Rai S., Luthra R. and Kumar S. 2003 Salt-tolerant mutants in glycophytic salinity response (GSR) genes in Catharanthus roseus. Theor. Appl. Genet. 106, 221–230.

    Google Scholar 

  • Pandey-Rai S., Mallavarupa G. R., Naqvi A. A., Yadav A., Rai S. K., Srivastava S. et al. 2006 Volatile compounds of leaves and flowers of periwinkle Catharanthus roseus (L.) G. Don from New Delhi. Flavour and Fragm. J. 21, 427–430.

    Article  CAS  Google Scholar 

  • Ratnaparkhe M. B., Tekeoglu M. and Muehlbauer F. J. 1998 Inter simple sequence repeat (ISSR) polymorphisms are useful for finding markers associated with disease resistance gene clusters. Theor. Appl. Genet. 97, 515–519.

    Article  CAS  Google Scholar 

  • Rischer H., Oresic M., Seppaenen-Laakso T., Katajamaa M., Lammertyn F., Ardiles-Diaz W. et al. 2006 Gene-to-metabolite networks for terpenoid indole alkaloid biosynthesis in Catharanthus roseus cells. Proc. Natl. Acad. Sci. USA 103, 5614–5619.

    Article  PubMed  CAS  Google Scholar 

  • Rubeena, Ford R. and Taylor P. W. J. 2003 Construction of an intraspecific linkage map of lentil (Lens culinaris ssp. culinaris). Theor. Appl. Genet. 107, 910–916.

    Article  PubMed  CAS  Google Scholar 

  • Saghai-Maroof M. A., Soliman K. M., Jorgensen R. A. and Allard R.W. 1984 Ribosomal DNA spacer-length polymorphism in barley: Mendelian inheritance, chromosomal location, and population dynamics. Proc. Natl. Acad. Sci. USA 81, 8014–8018.

    Article  PubMed  CAS  Google Scholar 

  • Shokeen B., Sethy N. K., Choudhary S. and Bhatia S. 2005 Development of STMS markers from the medicinal plant Madagascar periwinkle [Catharanthus roseus (L.) G. Don.]. Mol. Biol. Notes 5, 818–820.

    CAS  Google Scholar 

  • Shokeen B., Sethy N. K., Kumar S. and Bhatia S. 2007 Isolation and characterization of microsatellite markers for analysis of molecular variation in the medicinal plant Madagascar periwinkle [Catharanthus roseus (L.) G. Don.]. Plant Science 172, 441–451.

    Article  CAS  Google Scholar 

  • Simpson M. G. 2006 Plant systematics. Elsevier, Amsterdam, The Netherlands.

    Google Scholar 

  • Snoeijer W. 2001 International register of Catharanthus roseus. Leiden/Amsterdam Centre for Drug Research, Division of Pharmacogrosy, Leiden.

    Google Scholar 

  • van der Fits L. and Memelink J. 2001 The jasmonate inducible AP2/ERF-domain transcription factor ORCA3 activates gene expression via interaction with a jasmonate-responsive promoter element. Plant J. 25, 43–53.

    Article  PubMed  Google Scholar 

  • van der Fits L., Deakin E. A., Hoge J. H. and Memelink J. 2000 The ternary transformation system: constitutive VirG on a compatible plasmid dramatically increases Agrobacterium-mediated plant transformation. Plant Mol. Biol. 43, 495–502.

    Article  PubMed  Google Scholar 

  • van der Heijden R., Jacobs D. I., Snoeijer W., Hallard D. and Verpoorte R. 2004 The Catharanthus alkaloids: pharmacognosy and biotechnology. Curr. Med. Chem. 11, 607–628.

    Article  Google Scholar 

  • Voorrips R. E. 2002 MapChart: software for the graphical presentation of linkage maps and QTLs. J. Hered. 93, 77–78.

    Article  PubMed  CAS  Google Scholar 

  • Wang Z., Weber J. L., Zhong G. and Tanksley S. D. 1994 Survey of plant short tandem DNA repeats. Theor. Appl. Genet. 88, 1–6.

    CAS  Google Scholar 

  • Weeden N. F., Swiecicki W. K., Timmerman-Vaughan G. M., Ellis T. H. N. and Ambrose M. 1996 The current pea linkage map. Pisum Genet. 28, 1–4.

    Google Scholar 

  • Weising K., Nybom H., Wolff K. and Kahl G. 2005 DNA fingerprinting in plants: principles, methods, and applications, 2nd ed. CRC Press, Boca Raton, FL.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. National Institute for Plant Genome Research (NIPGR), Aruna Asaf Ali Marg, Post Box No. 10531, New Delhi, 110 067, India

    Sarika Gupta, Sashi Pandey-Rai, Suchi Srivastava, Manoj Prasad & Sushil Kumar

  2. School of Life Sciences, Pandit Ravi Shankar Shukla University, Raipur, 492 010, India

    Sarika Gupta & Subhas Chandra Naithani

Authors
  1. Sarika Gupta
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Sashi Pandey-Rai
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Suchi Srivastava
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Subhas Chandra Naithani
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Manoj Prasad
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Sushil Kumar
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Sushil Kumar.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Gupta, S., Pandey-Rai, S., Srivastava, S. et al. Construction of genetic linkage map of the medicinal and ornamental plant Catharanthus roseus . J Genet 86, 259–268 (2007). https://doi.org/10.1007/s12041-007-0033-8

Download citation

  • Received:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12041-007-0033-8

Keywords

Search

Navigation