Erratum to: Mol Neurobiol

DOI 10.1007/s12035-014-8738-1

The original version of the article unfortunately contains mistake on Fig. 2a as the upper image should be from the GFP group cells. This correction does not change the conclusion of the article. The authors are hereby correcting this figure as shown.

Fig. 2
figure 1

Overexpression of E46K mutant α-synuclein inhibits autophagy. induction. (a) PC12 cells stably overexpressing E46K mutant α-synuclein GFP were labeled with 50 nM of LysoTracker for 2 h and then visualized using a confocal microscopy. Scale bar 10 μm. PC12 cells stable overexpression of E46K mutant α-synuclein GFP (b) or PC12 and HEK293 cells transfected with E46K mutant α-synuclein (c) for 48 h were harvested, and protein extracts were immunoblotted for LC3. (d) Immunostaining of endogenous LC3 positive puncta in PC12 cells stably transfected with E46K mutant α-synuclein GFP. Scale bar 10 μm. (e) Analysis of autophagosome (indicated with a red arrow) numbers in PC12 cells stably overexpressing E46K mutant α-synuclein GFP by EM. Scale bar 500 nm. (f) PC12 cells stably and HEK293 cells transiently transfected with E46K mutant α-synuclein were lysted and immunoblotted with an anti-Atg 4B antibody. (g) PC12 cells stably overexpressing E46K mutant α-synuclein GFP were treated with and without 100 μMCQ for 2 h and then lysted and subjected to SDS-PAGE. (h) CHO cells stably expressing GFP-LC3 were transfected with E46K mutant α-synuclein or an empty vector for 48 h followed by 2 h of incubation with and without 100 μM CQ. GFP fluorescence intensity was assessed using flowcytometry. For all of the graphs, data were shown as means ± SD for three independent experiments performed in triplicate. *P < 0.05; **P < 0.01; ***P < 0.001 by two-tailed Student’s t test

Full size image