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Identification of Aluminum-Regulated Genes by cDNA-AFLP Analysis of Roots in Two Contrasting Genotypes of Highbush Blueberry (Vaccinium corymbosum L.)

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Abstract

To investigate the molecular mechanisms of Al3+-stress in blueberry, a cDNA-amplified fragment length polymorphism (cDNA-AFLP) analysis was employed to identify Al-regulated genes in roots of contrasting genotypes of highbush blueberry (Brigitta, Al3+-resistant and Bluegold, Al3+-sensitive). Plants grown in hydroponic culture were treated with 0 and 100 μM Al3+ and collected at different times over 48 h. Seventy transcript-derived fragments (TDFs) were identified as being Al3+ responsive, 31 of which showed significant homology to genes with known or putative functions. Twelve TDFs were homologous to uncharacterized genes and 27 did not have significant matches. The expression pattern of several of the genes with known functions in other species was confirmed by quantitative relative real-time RT-PCR. Twelve genes of known or putative function were related to cellular metabolism, nine associated to stress responses and other transcription and transport facilitation processes. Genes involved in signal transduction, photosynthetic and energy processes were also identified, suggesting that a multitude of processes are implicated in the Al3+-stress response as reported previously for other species. The Al3+-stress response genes identified in this study could be involved in Al3+-resistance in woody plants.

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Abbreviations

cDNA-AFLP:

DNA complementary to RNA-amplified fragment length polymorphism

TDF:

Transcript derived fragment

Aluminum:

Al3+

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Acknowledgments

This work was financially supported by the Fruit Consortium, 07Genoma01, the Millennium Nucleus for Plant Functional Genomics (P06-009-F), Fondecyt Project No. 11080231 and Fondecyt Project No. 1080372. C. Inostroza-Blancheteau was supported by a PhD fellowship from CONICYT-Chile and UFRO. F. Aquea is supported by a Postdoctoral Project “Programa Bicentenario de Ciencia y Tecnología/CONICYT-Banco Mundial” PSD74-2006. We thank Michael Handford for assistance in language support.

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  1. Center of Plant, Soil Interaction and Natural Resources Biotechnology, Scientific and Tecnological Bioresource Nucleus (BIOREN-UFRO), Universidad de La Frontera, Av. Francisco Salazar 01145, P.O. Box 54-D, Temuco, Chile

    Claudio Inostroza-Blancheteau, Marjorie Reyes-Díaz & Miren Alberdi

  2. Facultad de Ciencias Biológicas, Departamento de Genética Molecular y Microbiología, Pontificia Universidad Católica de Chile, Av. Alameda 340, P.O. Box 114-D, Santiago, Chile

    Claudio Inostroza-Blancheteau, Felipe Aquea & Patricio Arce-Johnson

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  1. Claudio Inostroza-Blancheteau
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Correspondence to Patricio Arce-Johnson.

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Inostroza-Blancheteau, C., Aquea, F., Reyes-Díaz, M. et al. Identification of Aluminum-Regulated Genes by cDNA-AFLP Analysis of Roots in Two Contrasting Genotypes of Highbush Blueberry (Vaccinium corymbosum L.). Mol Biotechnol 49, 32–41 (2011). https://doi.org/10.1007/s12033-010-9373-3

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