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Isolation and Characterization of Antioxidation Enzymes from Cells of Zedoary (Curcuma zedoaria Roscoe) Cultured in a 5-l Bioreactor

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Abstract

In this study, a cell suspension culture system for zedoary (Curcuma zedoaria Roscoe) was developed, using 50 g/l of fresh weight inoculum in a batch culture. The highest cell biomass obtained from a 5-l bioreactor equipped with three impellers after 14 days of culture was utilized to extract secondary metabolites (essential oil and curcumin) and determine the activities of antioxidant enzymes (peroxidase, superoxide dismutase, and catalase). For essential oil and curcumin, zedoary extracts were recovered via a variety of methods: steam distillation, volatile solvents, and Soxhlet. After 14 days of culture using volatile solvents, the optimal yield of essential oil (1.78%) was obtained when using petroleum ether at 40°C in 6 h of extraction, and the best curcumin yield (9.69%) was obtained at 60°C in 6 h via extraction with 90% ethanol. The activities of antioxidant enzymes from zedoary cells were also assessed. The specific activities of peroxidase, superoxide-dismutase, and catalase reached maximum values of 0.63 U/mg of protein, 16.60 U/mg of protein, and 19.59 U/mg of protein after 14 days of culture, respectively.

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Abbreviations

BAP:

Benzylamino purine

CAT:

Catalase

2,4-D:

2,4-Dichlorophenoxyacetic acid

EDTA:

Ethylenediaminotetraacetic acid

MS:

Murashige and Skoog

POD:

Peroxidase

SOD:

Superoxide dimutase

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Acknowledgments

This study was supported by a Korea Research Foundation Grant (KRF-2004-005-F00025) and a grant from the Basic Research Program in Natural Science of the Vietnamese Ministry of Science and Technology (2006-2008).

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Authors and Affiliations

  1. College of Sciences and Centre for Resource-Environment and Biotechnology, Hue University, Hue, Vietnam

    Nguyen-Hoang Loc, Doan-Thi-Hong Diem, Doan-Huu-Nhat Binh & Dao-Thi Huong

  2. Division of Biological Sciences and Research Center for Bioactive Materials, Chonbuk National University, Jeonju, Chonbuk, 561-756, Republic of Korea

    Tae-Geum Kim & Moon-Sik Yang

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  1. Nguyen-Hoang Loc
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Correspondence to Moon-Sik Yang.

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Loc, NH., Diem, DTH., Binh, DHN. et al. Isolation and Characterization of Antioxidation Enzymes from Cells of Zedoary (Curcuma zedoaria Roscoe) Cultured in a 5-l Bioreactor. Mol Biotechnol 38, 81–87 (2008). https://doi.org/10.1007/s12033-007-9014-7

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  • DOI: https://doi.org/10.1007/s12033-007-9014-7

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