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Dihydromyricetin Ameliorates 3NP-induced Behavioral Deficits and Striatal Injury in Rats

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Abstract

Oxidative stress is closely involved in neurodegenerative diseases. The present study aimed to examine the effect of anti-oxidant DHM (dihydromyricetin) on 3NP (3-nitropropionic acid) -induced behavioral deficits of experimental rats and striatal histopathological injury by using behavioral, imaging, biochemistry, histochemistry and molecular biology technologies. The experimental results showed that both motor dysfunctions and learning and memory impairments induced by 3NP were significantly reduced after DHM treatment. 3NP-induced striatal metabolic abnormality was also remarkably improved by DHM treatment, showed as the increased glucose metabolism in PET/CT scan, decreased MDA (malondialdehyde) and increased SOD (superoxide dismutase) activity in enzyme histochemical staining. In addition, the cell apoptosis was evidently detected in the striatum of the 3NP group, while in the 3NP + DHM group, the number of apoptotic cells was remarkably reduced. 3NP treatment obviously induced down-regulation of Bcl-2, and up-regulations of Bax and Cleaved Caspase-3, while these changes were significantly reversed by DHM treatment. The present results suggested that DHM showed its protective effect by anti-oxidant and anti-apoptosis mechanisms.

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Acknowledgments

This research was supported by the National Science Foundation of China (No. 81471288, 31070941 and 81301063), by the Major State Basic Research Development Program of China (973 Program, No. 2010CB530004) and by the Project 2016075 supported by SZU R/D Fund.

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    Authors and Affiliations

    1. Department of Anatomy, Zhongshan School of Medicine, Sun Yat-sen University, 74, Zhongshan Rd. 2, Guangzhou, 510080, China

      Youlan Li, Weiping Wang, Si Chen, Jiajia Wu, Lisi OuYang, Yaxi Zhu, Keyi Li, Mali Zhan, Zongwei Liu, Yu Jia, Yuxin Ma & Wanlong Lei

    2. College of Psychology and Sociology, Shenzhen University, Shenzhen, China

      Shuhua Mu

    3. Department of Anesthesiology, Guangdong No. 2 Provincial People’s Hospital, Guangdong Provincial Emergency Hospital, Guangzhou, China

      Bingbing Liu

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    1. Shuhua Mu
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    Correspondence to Wanlong Lei.

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    Shuhua Mu and Youlan Li contributed equally to this work.

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    Mu, S., Li, Y., Liu, B. et al. Dihydromyricetin Ameliorates 3NP-induced Behavioral Deficits and Striatal Injury in Rats. J Mol Neurosci 60, 267–275 (2016). https://doi.org/10.1007/s12031-016-0801-0

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