Abstract
The serum proteomic profiles of mice exposed to terrified-sound-induced stress and after stress release were investigated. Serum samples from 32 mice were divided into four groups (n = 8 each) and analyzed using matrix-assisted laser desorption and ionization time-of-flight mass spectrometry techniques (MALDI-TOF MS) combined with magnetic bead-based weak cation-exchange chromatography. ClinProTools software identified several distinct markers that differed between the stressed and control groups and between the stress released and stressed released controls. Of 33 m/z peaks that differed among the four groups, 17 were significantly different (P < 0.05). Five peaks (m/z: 2793.37, 2924.86, 1979.90, 3492.49, 3880.24) showed significant differences in expression after exposure to terrified-sound stress and returned to control levels after stress release. These were sequence identified as peptide regions of dimethylaniline monooxygenase, myosin-9, uncharacterized protein in Rattus norvegicus, apolipoprotein C-I, and plasma serine protease inhibitor (Serpina 5). Our study provides the first evidence of significant changes in serum proteomic profiles in mice exposed to terrified-sound stress, which suggests that protein expression profiles are affected by the stress. Normal expression levels were restored after stress release, suggesting the activation of self-adjustment mechanisms for the recovery of protein expression levels altered by this stress.
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Acknowledgments
This work was supported by the National Natural Science Foundation of China (grant number: 81200845) and the Key Science and Technology Program of Shaanxi province (grant number: 2015SF166).
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The authors declare that they have no competing interests.
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Juan Yang and Xin Zhang are co-first authors.
Juan Yang and Xin Zhang contributed equally to this work.
This article is retracted as consented by the authors, Editor-in-Chief and publisher for the following reason: The human and mouse samples were mixed (by mistake) and the results shown for the mouse are actually human sequences. The authors regret the mistake and apologize to the readers.
An erratum to this article can be found online at http://dx.doi.org/10.1007/s12031-016-0816-6.
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Yang, J., Zhang, X., Xiong, X. et al. RETRACTED ARTICLE: Effects of a Terrified-Sound Stress on Serum Proteomic Profiling in Mice. J Mol Neurosci 57, 211–218 (2015). https://doi.org/10.1007/s12031-015-0607-5
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DOI: https://doi.org/10.1007/s12031-015-0607-5