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Combining Bone Marrow Stromal Cells with Green Tea Polyphenols Attenuates the Blood-Spinal Cord Barrier Permeability in Rats with Compression Spinal Cord Injury

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Abstract

This study was performed to investigate the effect of bone marrow stromal cells (BMSCs) combined with green tea polyphenols (GTPs) on the blood-spinal cord barrier (BSCB) permeability after spinal cord injury (SCI) in the rat model. In the model of SCI rats, we found that the water content and the BSCB permeability were decreased by BMSCs and GTPs treatment, and their combination had a synergistic effect. Further, the motor function of rats was also greatly improved by BMSCs and GTPs administration. After treated by the combination of BMSCs and GTPs, SCI rats showed the up-regulated expression of tight junction (TJ) associated proteins claudin-5, occludin and ZO-1 by Western blot, which was more remarkable than that in the single treatment. The increased expression levels of claudin-5, occludin, and ZO-1 were the most obvious in the spinal cord microvessels using immunohistochemistry assay. This led to the conclusion that the combination of BMSCs and GTPs could decrease the BSCB permeability by up-regulating protein expression levels of claudin-5, occludin, and ZO-1. In addition, after BMSCs and GTPs administration, the results of Western blot and enzyme-linked immunosorbent assay (ELISA) revealed a significant decrease in protein expression level and the activation of nuclear factor-кB (NF-кB) p65. Our results indicated that combination of BMSCs and GTPs could improve motor function after SCI, which might be correlated with improvements in BSCB integrity, and that NF-кB might be involved in the modulating process.

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Acknowledgments

This work was supported by Natural Science Foundation of China (Nos. 81171799, 81272074, and 81471853), Doctoral Scientific Research Starting Foundation of Liaoning Province (No. 20121096), Program for Liaoning Excellent Talents in University (No. LR2013091), Scientific Research Starting Foundation for PHD and Returned Overseas Teacher of Liaoning Medical University (No. Y2012B011), and Special Funds for Clinical Medicine Construction of the Principal’s Fund of the Liaoning Medical University (No. XZJJ20130241).

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The authors declare that there are no conflicts to disclose.

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Authors and Affiliations

  1. Department of Orthopedics, The First Affiliated Hospital, Liaoning Medical University, Jinzhou, 121001, People’s Republic of China

    De-shui Yu, Yang Cao, Yan-song Wang, Yun-long Bi, Zi-jian Wei, Song-ming Tong, Gang Lv & Xi-fan Mei

  2. Department of Neurobiology, College of Basic Medicine, China Medical University, Shenyang, 110001, People’s Republic of China

    Li-bo Liu

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  1. De-shui Yu
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  9. Xi-fan Mei
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Correspondence to Xi-fan Mei.

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Yu, Ds., Liu, Lb., Cao, Y. et al. Combining Bone Marrow Stromal Cells with Green Tea Polyphenols Attenuates the Blood-Spinal Cord Barrier Permeability in Rats with Compression Spinal Cord Injury. J Mol Neurosci 56, 388–396 (2015). https://doi.org/10.1007/s12031-015-0564-z

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  • DOI: https://doi.org/10.1007/s12031-015-0564-z

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