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The effect of chronic kisspeptin administration on seminal fructose levels in male mice

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Abstract

The discovery that kisspeptin was critical for normal fertility in all mammalian species including humans, ushered in a new chapter in our understanding of the control of GnRH secretion. Kisspeptin, the product of the KISS1 gene, plays an essential role in the regulation of spermatogenesis acting primarily at the hypothalamic level of the gonadotropic axis. Among the many identified substances in human semen, fructose is becoming increasingly significant. Fructose is synthesized and secreted by the seminal vesicles. Its synthesis is regulated by androgens and it is correlated directly with the levels of testosterone. Dose dependent degeneration of seminal vesicle has been described following intraperitoneal kisspeptin treatment; however, effects of kisspeptin administration on the levels of seminal fructose remain elusive till date. The present study, therefore, addresses the effects of 12-day administration of kisspeptin on seminal fructose levels in male mice. Kisspeptin-10 was administered intraperitoneally at different dosage concentrations (1 μg, 1 ng, and 10 ρg) to adult male mice, twice daily for 12 days. Seminal fructose levels were studied photometrically after 12 days of treatment. At the end of the treatment, seminal fructose levels decreased significantly after all tested doses. Chronic intermittent kisspeptin-10 administration negatively regulates seminal fructose levels in adult male mice.

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Acknowledgments

The study was funded by the startup research grant provided to the corresponding author by the Higher Education Commission (HEC), H-9, Islamabad, Pakistan. The authors are indebted to Dr. Muhammad Aslam Khan, Clinical Pathologist, Department of Pathology, District Headquarter Teaching Hospital, Dera Ismail Khan for technical assistance.

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The authors declare that they have no competing interests.

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Authors and Affiliations

  1. Gomal Centre for Biochemistry and Biotechnology, Gomal University, Dera Ismail Khan, 29050, Pakistan

    Faiqah Ramzan & Muhammad Ayaz Khan

  2. Department of Physiology, Institute of Basic Medical Sciences, Khyber Medical University, Peshawar, Pakistan

    Muhammad Haris Ramzan

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  1. Faiqah Ramzan
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  2. Muhammad Ayaz Khan
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Correspondence to Faiqah Ramzan.

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Ramzan, F., Khan, M.A. & Ramzan, M.H. The effect of chronic kisspeptin administration on seminal fructose levels in male mice. Endocrine 45, 144–147 (2014). https://doi.org/10.1007/s12020-013-0016-x

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  • DOI: https://doi.org/10.1007/s12020-013-0016-x

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