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Epigallocatechin-3-Gallate Protects Na+ Channels in Rat Ventricular Myocytes Against Sulfite

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Abstract

Sulfite (bisulfite/sulfite) can affect voltage-gated sodium (Na+) channels (VGSC) in a concentration-dependent manner in isolated rat ventricular myocytes. In this study, the effect of epigallocatechin-3-gallate (EGCG) on VGSC in isolated ventricular myocytes was studied. Ventricular myocytes were exposed to 10 μM bisulfite/sulfite for 10 min, and EGCG was then administered in different concentrations (10, 30, 50 μg ml−1). Decreased activity of superoxide dismutase, catalase (CAT) and glutathione peroxidase (GPx) was observed after bisulfite/sulfite exposure, with significant increase in Na+ currents (I Na) and alterations in half-activation voltage and half-inactivation voltage. Intracellular reactive oxygen species (ROS) such as hydrogen peroxide (H2O2), hydroxyl (OH·), and superoxide anion (O ·−2 ) were increased. After EGCG treatment, activity of the aforementioned enzymes increased while the ROS level decreased. The effects progressed with increasing amounts of EGCG, up to a level similar to blank control at the dose of 50 μg ml−1 EGCG, EGCG also reduced the I Na and reversed the alterations in half-activation voltage and half-inactivation voltage. In conclusion, EGCG could protect Na+ channels in rat ventricular myocytes against the oxidative damage induced by sulfite as a scavenger of the ROS.

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Authors and Affiliations

  1. College of Environmental Science and Resources, Shanxi University, Taiyuan, China

    Haiying Wei

  2. Institute of Environmental Medicine and Toxicology, Shanxi University, 92 Wucheng Road, 030006, Taiyuan, China

    Ziqiang Meng

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  1. Haiying Wei
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  2. Ziqiang Meng
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Correspondence to Ziqiang Meng.

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Wei, H., Meng, Z. Epigallocatechin-3-Gallate Protects Na+ Channels in Rat Ventricular Myocytes Against Sulfite. Cardiovasc Toxicol 10, 166–173 (2010). https://doi.org/10.1007/s12012-010-9075-x

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  • DOI: https://doi.org/10.1007/s12012-010-9075-x

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