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Astilbin Attenuates Cadmium-Induced Adipose Tissue Damage by Inhibiting NF-κB Pathways and Regulating the Expression of HSPs in Chicken

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Abstract

Cadmium (Cd) can damage tissues by inducing oxidative stress, lymphocyte infiltration, and inflammation in these sites. Meanwhile, astilbin (Ast) is an antioxidant agent. At present, only a few mechanisms of Cd-induced adipose tissue damage have been described. Herein, we assessed the potential protective effects and the molecular mechanism underlying the antioxidant properly of Ast after Cd intake in chicken adipose tissue. In this study, a total of 160 7-day-old roosters were randomly divided into four groups. Roosters were fed with a basic diet (C group), Ast 40 mg/kg (Ast group), CdCl2 150 mg/kg + Ast 40 mg/kg (Cd/Ast group), and CdCl2 150 mg/kg (Cd group) for 60 days. We found that Cd intake changed the morphology and structure of adipose tissues and decreased the expression of several antioxidants, including total superoxide dismutase (T-SOD), glutathione peroxidase (GSH-Px), catalase (CAT), and total antioxidant capacity (T-AOC), but increased those of oxidative stress markers including malondialdehyde (MDA), inducible nitric oxide synthase (iNOS), NO, and H2O2. Cd further activated the nuclear factor kappa B (NF-κB) signaling pathway and increased the expression of the inflammation-related mediators, interleukin 1beta (IL-1β), interleukin 6 (IL-6), interleukin 8 (IL-8), interleukin 10 (IL-10), cyclooxygenase-2 (COX-2), iNOS, prostaglandin E synthase (PTGES), tumor necrosis factor-alpha (TNF-α), and interferon-gamma (IFN-γ). Cd-induced oxidative stress upregulated the expression of three heat shock proteins (HSPs), including HSP27, HSP70, and HSP90. Summarily, Cd causes oxidative stress-mediated tissue damage by activating the NF-κB pathway, promoting inflammation and upregulating the expression of HSPs. However, Ast supplementation modulates oxidative stress in adipose tissue by inhibiting inflammation mediated by the NF-κB pathway and regulating the expression of HSPs.

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Data Availability

The authors declare that the data supporting the findings of this study are available within the article.

Abbreviations

Cd:

Cadmium

Ast:

Astilbin

cDNA:

Complementary DNA

GAPDH:

Glyceraldehyde 3-phosphate dehydrogenase

HSPs:

heat shock proteins

NF-κB:

Nuclear factor kappa B

TBST:

Tris-buffered saline-Tween 20

IL-1β:

Interleukin 1beta

IL-6:

Interleukin 6

IL-8:

Interleukin 8

IL-10:

Interleukin 10

TNF-α:

Tumor necrosis factor-alpha

IFN-γ:

Interferon-gamma

COX-2:

Cycooxygenase-2

iNOS:

Inducible nitric oxide synthase

T-SOD:

Total superoxide dismutase

GSH-Px:

Glutathione peroxidase

CAT:

Catalase

T-AOC:

Total antioxidant capacity

MDA:

Malondialdehyde

PTGES:

Prostaglandin E synthase

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Acknowledgements

We thank the members of the Chinese veterinary laboratory at the College of Veterinary Medicine, Northeast Agricultural University, for helping us to collect the samples. Our sincere gratitude to the Heilongjiang Key Laboratory for Laboratory Animals and Comparative Medicine.

Funding

This study was funded by the National Natural Science Foundation of China (No.31702282), the “Academic backbone” Project of Northeast Agricultural University (No. 20XG32), the University Nursing Program for Young Scholars with Creative Talents in Heilongjiang Province (UNPYSCT-2016139), the Northeast Agricultural University Student Innovation Practical Training project (No. 202010224076), the “Young Talents” Project of Northeast Agricultural University (No.18QC41), and the Northeast Agricultural University/Scientific Observing and Experimental Station of Animal Nutrition and Feed Science in Northeast, Ministry of Agriculture. P.R. China (yy-2017–09).

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Authors and Affiliations

  1. College of Veterinary Medicine, Northeast Agricultural University, Harbin, 150030, China

    Jianxu Sun, Zitao Jiao, Weifeng Zhu, Xiuyu Li, Panpan Wang, Jiangfeng Wang, Tiange Tai, Yuxi Wang, Haibin Wang & Guangliang Shi

  2. Key Laboratory of the Provincial Education, Department of Heilongjiang for Common Animal Disease Prevention and Treatment, Harbin, China

    Jianxu Sun, Zitao Jiao, Weifeng Zhu, Xiuyu Li, Panpan Wang, Jiangfeng Wang, Tiange Tai, Yuxi Wang, Haibin Wang & Guangliang Shi

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Contributions

Jianxu Sun: conceptualization, data curation, writing-original draft. Zitao Jiao: searching literature, writing-original draft. Xiuyu Li: experiment. Weifeng Zhu: Experiment. Panpan Wang: experiment. Jiangfeng Wang: experiment. Tiange Tai: experiment. Yuxi Wang: experiment. Haibin Wang: writing-Reviewing. Guangliang Shi: methodology, financial support, writing-reviewing.

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Correspondence to Guangliang Shi.

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All procedures used in this study were approved by the Institutional Animal Care and Use Committee of Northeast Agricultural University. This article does not contain any studies with human performed by any of the authors.

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Sun, J., Jiao, Z., Zhu, W. et al. Astilbin Attenuates Cadmium-Induced Adipose Tissue Damage by Inhibiting NF-κB Pathways and Regulating the Expression of HSPs in Chicken. Biol Trace Elem Res 201, 2512–2523 (2023). https://doi.org/10.1007/s12011-022-03327-y

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