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Western Blotting: An Introduction

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Western Blotting

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1312))

Abstract

Western blotting is an important procedure for the immunodetection of proteins, particularly proteins that are of low abundance. This process involves the transfer of protein patterns from gel to microporous membrane. Electrophoretic as well as non-electrophoretic transfer of proteins to membranes was first described in 1979. Protein blotting has evolved greatly since the inception of this protocol, allowing protein transfer to be accomplished in a variety of ways.

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Author information

Authors and Affiliations

  1. Arthritis and Clinical Immunology, Oklahoma Medical Research Foundation, 825 NE 13th Street, Oklahoma City, OK, 73104, USA

    Biji T. Kurien & R. Hal Scofield

  2. Department of Medicine, University of Oklahoma Health Sciences Center, Oklahoma City, OK, 73104, USA

    Biji T. Kurien & R. Hal Scofield

  3. Department of Veterans Affairs Medical Center, Oklahoma City, OK, 73104, USA

    Biji T. Kurien & R. Hal Scofield

Authors
  1. Biji T. Kurien
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  2. R. Hal Scofield
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Corresponding author

Correspondence to Biji T. Kurien .

Editor information

Editors and Affiliations

  1. Department of Medicine, University of Oklahoma, Arthritis and Clinical Immunology, Oklahoma Medical Research Foundation, Health Sciences Center, Department of Veterans, Oklahoma City, Oklahoma, USA

    Biji T. Kurien

  2. Department of Medicine, Arthritis and Clinical Immunology, Oklahoma Medical Research Foundation, Health Sciences Center, Department of Veterans Affairs Medical Center, Oklahoma City, Oklahoma, USA

    R. Hal Scofield

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Kurien, B.T., Scofield, R.H. (2015). Western Blotting: An Introduction. In: Kurien, B., Scofield, R. (eds) Western Blotting. Methods in Molecular Biology, vol 1312. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2694-7_5

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  • DOI: https://doi.org/10.1007/978-1-4939-2694-7_5

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-2693-0

  • Online ISBN: 978-1-4939-2694-7

  • eBook Packages: Springer Protocols

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