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Selenium-Alleviated Hepatocyte Necrosis and DNA Damage in Cyclophosphamide-Treated Geese by Mitigating Oxidative Stress

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Abstract

Selenium (Se) has been well recognized as an immune-enhancing agent with antioxidant and anti-tumor properties. The commonly used chemotherapy drug, cyclophosphamide (CTX), induces liver injury by increasing the reactive oxygen species (ROS) level. However, little is known about how Se alleviates CTX-induced liver injury in geese. In this study, 90 male Magang geese (3 days old) were randomly allocated into three groups (control, CTX, and Se + CTX group) with three replicates per group and ten geese per replicate. The control and CTX groups were fed a basal diet (Se content was 0.03 mg/kg). The Se + CTX group was fed a basal diet containing 0.44 mg/kg sodium selenite (Se content was 0.2 + 0.03 mg/kg). The control group was injected with 0.5 mL saline, while the CTX and Se + CTX groups were injected with CTX at 40 mg/kg body weight per day on days 21–23. The liver index, liver histology, and ultra-micromorphology detected antioxidant enzyme activity in the liver and serum. In addition, we detected the liver marker enzymes and protein levels in serum, and hepatocyte DNA damage. Se could alleviate liver development dysregulation, hepatocyte structural damage, the disturbances in antioxidant enzyme (GPx, CAT, and SOD) activity, and malondialdehyde (MDA) levels in the serum and liver. Besides, Se could alleviate the dysregulation of liver marker enzyme (ALT and AST) activity and protein (ALB and TP) levels in the serum, and DNA migration induced by CTX. In conclusion, Se may inhibit hepatocyte necrosis and DNA damage by inhibiting CTX-induced oxidative stress.

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Acknowledgements

We appreciate the support of Guangdong Province Key Laboratory of Waterfowl Healthy Breeding, Guangzhou 510225, China.

Funding

This work was supported by the National Key Technologies R&D Program of China (No. 2016YFD0501605), Major Research Project in Universities of Guangdong Province (No. 2017KZDXM046), Inter-governmental S & T Exchanges Project between China and Belarus (No. CB01-13), and Science & Technology Planning Project of Guangzhou (No. 201604020061).

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  1. Bingxin Li and Wanyan Li contributed equally to this work.

Authors and Affiliations

  1. Zhongkai University of Agriculture and Engineering, Guangzhou, China

    Bingxin Li, Wanyan Li, Yunbo Tian, Sixuan Guo, Long Qian, Danning Xu & Nan Cao

  2. Guangdong Province Key Laboratory of Waterfowl Healthy Breeding, Guangzhou, China

    Wanyan Li, Yunbo Tian, Sixuan Guo, Long Qian, Danning Xu & Nan Cao

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  1. Bingxin Li
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  2. Wanyan Li
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Correspondence to Danning Xu or Nan Cao.

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Li, B., Li, W., Tian, Y. et al. Selenium-Alleviated Hepatocyte Necrosis and DNA Damage in Cyclophosphamide-Treated Geese by Mitigating Oxidative Stress. Biol Trace Elem Res 193, 508–516 (2020). https://doi.org/10.1007/s12011-019-01717-3

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