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Cardioprotective Effect of Selenium Against Cyclophosphamide-Induced Cardiotoxicity in Rats

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Abstract

The objective of this study is to evaluate the possible protective effects of selenium (Se) against cyclophosphamide (CP)-induced acute cardiotoxicity in rats. A total of 42 male Spraque-Dawley rats were divided into six groups (n = 7). Rats in the first group were served as control. Rats in the second group received CP (150 mg/kg) at the sixth day of experiment. Animals in the third and fourth groups were treated with only 0.5 and 1 mg/kg Se respectively for six consecutive days. Rats in the fifth and sixth groups received respective Se doses (0.5 or 1 mg/kg) for 6 days and then a single dose of CP administered on the sixth day. On day 7, the animals were sacrificed; blood samples were collected to measure malondialdehyde (MDA), glutathione (GSH), lactate dehydrogenase (LDH), creatine kinase-MB (CK-MB), and ischemia-modified albumin (IMA) levels. Heart tissues were processed routinely and tissue sections were stained with H + E for light microscopic examination. In the CP-treated rats MDA, LDH, CK-MB, and IMA serum levels increased, while GSH levels decreased. Microscopic evaluation showed that tissue damage was conspicuously lower in CP plus Se groups. Moreover, 1 mg/kg Se was more protective than 0.5 mg/kg Se as indicated by histopathological and biochemical values. In conclusion, Se is suggested to be a potential candidate to ameliorate CP-induced cardiotoxicity which may be related to its antioxidant activity.

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Acknowledgments

This study was financially supported by grants (P.N 2014-254) from the Eskisehir Osmangazi University Scientific Research Project, Turkey.

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Authors and Affiliations

  1. Faculty of Art and Science, Department of Biology, Eskisehir Osmangazi University, 26480, Eskisehir, Turkey

    Sibel Gunes, Pinar Karasati & Adnan Ayhanci

  2. Medical Faculty, Department of Histology and Embryology, Eskisehir Osmangazi University, 26480, Eskisehir, Turkey

    Varol Sahinturk

  3. Vocational School of Health Service, Medical Services and Techniques Department, Kırıkkale University, Kırıkkale, Turkey

    Ilknur Kulcanay Sahin

Authors
  1. Sibel Gunes
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  2. Varol Sahinturk
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  3. Pinar Karasati
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  5. Adnan Ayhanci
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Corresponding author

Correspondence to Sibel Gunes.

Ethics declarations

The experiments were conducted in accordance with the Guide for the Care and Use of Laboratory Animals prepared by the National Academy of Sciences (1996) and approved by Eskisehir Osmangazi University Institutional Animal Ethics Committee (Protocol no 14.01.2014-254/2014).

Conflict of Interest

The authors declare that they have no competing interests.

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Gunes, S., Sahinturk, V., Karasati, P. et al. Cardioprotective Effect of Selenium Against Cyclophosphamide-Induced Cardiotoxicity in Rats. Biol Trace Elem Res 177, 107–114 (2017). https://doi.org/10.1007/s12011-016-0858-1

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  • DOI: https://doi.org/10.1007/s12011-016-0858-1

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