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Ameliorative Effects of Selenium on Cadmium-Induced Injury in the Chicken Ovary: Mechanisms of Oxidative Stress and Endoplasmic Reticulum Stress in Cadmium-Induced Apoptosis

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Abstract

Despite the well-established toxicity of cadmium (Cd) to animals and the ameliorative effects of selenium (Se), some specific mechanisms in the chicken ovary are not yet clarified. To explore the mechanism by which the toxicity effect of Cd is induced and explore the effect of supranutritional Se on Cd toxicity in female bird reproduction, forty-eight 50-day-old Isa Brown female chickens were divided randomly into four groups. Group I (control group) was fed the basic diet containing 0.2 mg/kg Se. Group II (Se-treated group) was fed the basic diet supplemented with sodium selenite (Na2SeO3), and the total Se content was 2 mg/kg. Group III (Se + Cd-treated group) was fed the basic diet supplemented with Na2SeO3; the total Se content was 2 mg/kg, and it was supplemented with 150 mg/kg cadmium chloride (CdCl2). Group IV (Cd-treated group) was with the basic diet supplemented with 150 mg/kg CdCl2. The Cd, estradiol (E2), and progestogen (P4) contents changed after subchronic Cd exposure in chicken ovarian tissue; subsequently, oxidative stress occurred and activated the endoplasmic reticulum (ER) pathway to induce apoptosis. Further, Se decreased the accumulation of Cd in ovarian tissue, increased the E2 and P4 contents, alleviated oxidative stress, and reduced apoptosis via the ER stress pathway. The present results demonstrated that Cd could induce apoptosis via the ER stress pathway in chicken ovarian tissue and that Se had a significant antagonistic effect. These results are potentially valuable for finding a strategy to prevent Cd poisoning.

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Abbreviations

Cd:

cadmium

Se:

selenium

E2:

estradiol

P4:

progestogen

Na2SeO3 :

sodium selenite

CdCl2 :

cadmium chloride

ER:

endoplasmic reticulum

SOD:

superoxide dismutase

GSH-Px:

glutathione peroxidase

MDA:

malondialdehyde

NO:

nitric oxide

iNOS:

inducible nitric oxide synthase

LPO:

lipid peroxidation

ROS:

reactive oxygen species

GRP78:

glucose-regulated protein 78

PERK:

PKR-like ER kinase

ATF6:

activating transcription factor 6

ATF4:

activating transcription factor 4

IRE1:

inositol requiring enzyme 1

Caspase:

cysteine-aspartic protease

CHOP:

ATF6-CCAAT/enhancer-binding protein-homologous protein

XBP1:

IRE1-X-box-binding protein 1

TRAF2:

IRE1-TNF receptor associated factor 2

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Acknowledgments

The authors thank the Key Laboratory of the Provincial Education Department of Heilongjiang for Common Animal Disease Prevention and Treatment.

Funding

This study was supported by the National Natural Science Foundation of China (Grant No.31472161).

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Authors and Affiliations

  1. College of Veterinary Medicine, Northeast Agricultural University, Harbin, 150030, People’s Republic of China

    Na Wan, Zhe Xu, Tianqi Liu, Yahong Min & Shu Li

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  1. Na Wan
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  2. Zhe Xu
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  3. Tianqi Liu
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  4. Yahong Min
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  5. Shu Li
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Correspondence to Shu Li.

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Humane Care of Animals

The experiments were approved by the Institutional Animal Care and Use Committee of the Northeast Agricultural University under the approved protocol number SRM-06.

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All of the authors have read the manuscript and agreed to submit it in its current form for consideration for publication in the Biological Trace Element Research.

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Wan, N., Xu, Z., Liu, T. et al. Ameliorative Effects of Selenium on Cadmium-Induced Injury in the Chicken Ovary: Mechanisms of Oxidative Stress and Endoplasmic Reticulum Stress in Cadmium-Induced Apoptosis. Biol Trace Elem Res 184, 463–473 (2018). https://doi.org/10.1007/s12011-017-1193-x

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  • DOI: https://doi.org/10.1007/s12011-017-1193-x

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