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Ameliorative Effect of Selenomethionine on Cadmium-Induced Hepatocyte Apoptosis via Regulating PI3K/AKT Pathway in Chickens

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Abstract

Selenium (Se) is a trace element for human and animal health. Cadmium (Cd) is a known human carcinogen. The effects of Cd on the environment and humans are well known. Because chickens are at the top of the food chain, it is a good experimental animal model for assessing heavy metal toxicity and its potential threat to humans. Selenomethionine (Se-met) is a suitable form for nutritional Se supplementation. Therefore, the toxicity of Cd to the chicken liver and the antagonistic effects of Se-met on Cd were examined at the molecular level in the present study. The results showed that oxidative stress indicators (apoptosis-related genes, P13K/AKT pathway–related genes, and heat shock proteins (HSPs)–related genes) in the Cd group have changed significantly, indicating Cd induced hepatocyte stress and apoptosis. Interestingly, the changes in oxidative stress indicators (apoptosis-related genes, P13K/AKT pathway–related genes, and HSPs-related genes) in the Cd-Se-met group were mitigated compared with the control group. Our results indicated that Cd can induce hepatocyte apoptosis and stress in the chickens. Se-met has an ameliorative effect on Cd-induced apoptosis of chicken hepatocyte by regulating PI3K/AKT pathway. Our findings will provide a new insight for better understanding of the detoxification function of Se-met to heavy metals.

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Funding

The study was financially supported by the Project Fund of Academic Cadre of Northeast Agricultural University (Project No. 700-507001).

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Authors and Affiliations

  1. College of Veterinary Medicine, Northeast Agricultural University, 59 Mucai Street, Harbin, 150030, China

    Xiaoyu Xiong & Shiwen Xu

  2. College of Animal Science and Technology, Northeast Agricultural University, 59 Mucai Street, Harbin, 150030, China

    Yu Zhang & Houjuan Xing

  3. Heilongjiang Agricultural and Rural Department, 4-1 Wenfu Street, Harbin, 150060, China

    Yu Zhang

  4. Key Laboratory of Animal Cellular and Genetic Engineering of Heilongjiang Province, Northeast Agricultural University, 59 Mucai Street, Harbin, 150030, China

    Shiwen Xu

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  1. Xiaoyu Xiong
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  2. Yu Zhang
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  4. Shiwen Xu
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Correspondence to Houjuan Xing or Shiwen Xu.

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All experiments were approved by the Institutional Animal Care and Use Committee of Northeast Agricultural University.

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Xiong, X., Zhang, Y., Xing, H. et al. Ameliorative Effect of Selenomethionine on Cadmium-Induced Hepatocyte Apoptosis via Regulating PI3K/AKT Pathway in Chickens. Biol Trace Elem Res 195, 559–568 (2020). https://doi.org/10.1007/s12011-019-01858-5

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