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Dental Fluorosis and Catalase Immunoreactivity of the Brain Tissues in Rats Exposed to High Fluoride Pre- and Postnatally

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Abstract

This study evaluated dental fluorosis of the incisors and immunoreactivity in the brain tissues of rats given chronic fluoride doses pre- and postnatally. Female rats were given drinking water with 0, 30 or 100 ppm fluoride ad libitum throughout gestation and the nursing period. In addition, 63 male offspring were treated with the same water regimens as the mothers after weaning and were followed for 1, 3 or 5 months. The upper and lower incisors were collected, and all teeth were examined under a stereomicroscope and scored by two blinded examiners using a modified rodent enamel fluorosis index. Cortical, hippocampal and cerebellar brain samples were evaluated morphologically and immunohistochemically. All fluoride-treated pups were born with low body weight (p = 0.001). All animals from the fluoride groups had enamel fluorosis with defects of various degrees. The increase in the dental fluorosis scores in the fluoride treatment groups was significant (p < 0.01). The catalase immunoreactivity in the 30- and 100-ppm fluoride groups was significantly higher than that in the controls after 1, 3 and 5 months (p < 0.001). In conclusion, this study showed that rats with dental fluorosis had catalase immunoreactivity in the brain tissues, which may reflect the neurobehavioral toxicity of fluoride.

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Acknowledgments

This study was supported by The Commission of Scientific Research Projects, Marmara University, Istanbul (Project number: SAG-C-DRP-031210-0275). The English language in this document has been checked by at least two professional editors, both native speakers of English. For a certificate, please see http://www.textcheck.com/certificate/PTnaCV

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Authors and Affiliations

  1. Department of Pediatric Dentistry, Faculty of Dentistry, Trakya University, Balkan Campus, 22030, Edirne, Turkey

    Şirin Güner

  2. Department of Pathology, Institute for Neurological Sciences, Marmara University, Istanbul, Turkey

    Süheyla Uyar-Bozkurt

  3. Department of Pediatric Dentistry, Faculty of Dentistry, Marmara University, Basibuyuk Campus, Basibuyuk, Maltepe, 34854, Istanbul, Turkey

    Eda Haznedaroğlu & Ali Menteş

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  1. Şirin Güner
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  2. Süheyla Uyar-Bozkurt
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  4. Ali Menteş
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Correspondence to Ali Menteş.

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Funding

This study was funded by SAG-C-DRP-031,210-0275.

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The authors declare that they have no conflict of interest.

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Güner, Ş., Uyar-Bozkurt, S., Haznedaroğlu, E. et al. Dental Fluorosis and Catalase Immunoreactivity of the Brain Tissues in Rats Exposed to High Fluoride Pre- and Postnatally. Biol Trace Elem Res 174, 150–157 (2016). https://doi.org/10.1007/s12011-016-0695-2

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  • DOI: https://doi.org/10.1007/s12011-016-0695-2

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