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Evaluation of caspase-dependent apoptosis during fluoride-induced liver lesion in pigs

  • Organ Toxicity and Mechanisms
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Abstract

Sixteen barrows (Duroc×Landrace×Yorkshire) were randomly divided into two groups, each consisting eight pigs. The groups received the same basal diet supplemented with 0 and 400 mg/kg fluoride, respectively. Histological examinations, including in situ terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL), Haematoxylin and Eosin staining (HE) and transmission electron microscopy observation, found apoptotic hepatocytes 50 days after additional 400 mg/kg fluoride treatment. The obvious DNA ladder and the significantly increased both hepatic caspase-9 and caspase-3 activity indicated that fluoride induced caspase-dependent apoptosis in vivo. In addition, serum glutamate pyruvate transaminase (GPT) activity and hepatic lipid peroxides (LPO) concentration was significantly increased. The activity of serum glutamate oxaloacetate transaminase (GOT) showed an increased trend. The results suggest that fluoride induces apoptosis by elevating the oxidative stress-induced lipid peroxidation, causing mitochondrial dysfunction and further activating caspase-9 and caspase-3.

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Acknowledgements

We thank Hongxiang Sun for his valuable suggestions. We also thank Jianyi Sun and Junyi Shi for their skilled technical assistance. The financial support provided by the National Basic Research Program of China (Project 2004CB117505) is gratefully acknowledged.

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Authors and Affiliations

  1. Feed Science Institute, College of Animal Science, Zhejiang University, No. 268, Kaixuan Road, HangZhou, 310029, People’s Republic of China

    Xiu An Zhan, Min Wang, Zi Rong Xu, Wei Fen Li & Jian Xin Li

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  1. Xiu An Zhan
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  2. Min Wang
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  3. Zi Rong Xu
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  4. Wei Fen Li
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  5. Jian Xin Li
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Correspondence to Min Wang.

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The name of the laboratory where the work was carried out: Feed Science Institute in Zhejiang university

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Zhan, X.A., Wang, M., Xu, Z.R. et al. Evaluation of caspase-dependent apoptosis during fluoride-induced liver lesion in pigs. Arch Toxicol 80, 74–80 (2006). https://doi.org/10.1007/s00204-005-0019-3

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  • DOI: https://doi.org/10.1007/s00204-005-0019-3

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