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Proteomic Analysis of Hippocampus in Offspring Male Mice Exposed to Fluoride and Lead

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Abstract

Fluoride and lead are two common pollutants in the environment. Previous investigations have found that high fluoride exposure can increase the lead burden. In this experiment, in order to study on the molecular mechanisms of central neural system injury induced by the above two elements, differently expressed protein spots in hippocampus of male mice treated with 150 mg sodium fluoride/L and/or 300 mg lead acetate/L in their drinking water were detected by two-dimensional electrophoresis (2-DE) and mass spectrometry (MS). The behavior tests showed that 56 days of fluoride and lead administration significantly reduced the vertical activity and lowered the memory ability of mice. In addition, results of 2-DE and MS revealed that nine spots demonstrated above a twofold change in the same trend in all treatment groups, which were mainly related with (1) energy metabolism, (2) cell stress response/chaperones, (3) cytoskeleton development, (4) protein metabolism, and (5) cell surface signal transduction. The findings could provide potential biomarkers for lesion in nervous system induced by fluoride and lead exposure.

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Acknowledgments

This research was supported by the China National Natural Science Foundation (Grant Nos. 31101869, 31201965, and 31172376), Shanxi Province Natural Science Foundation (Grant No. 2012021027–5), and Program for the Top Young Academic Leaders of Higher Learning Institutions of Shanxi.

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Authors and Affiliations

  1. College of Animal Science and Technology, Shanxi Agricultural University, Taigu, Shanxi, China

    Ruiyan Niu, Shuangling Liu, Jinming Wang, Jianhai Zhang, Zilong Sun & Jundong Wang

  2. Shanxi Key Laboratory of Environmental Veterinary Medicine, Shanxi Agricultural University, Taigu, Shanxi, China

    Ruiyan Niu, Jinming Wang, Jianhai Zhang, Zilong Sun & Jundong Wang

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  1. Ruiyan Niu
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  2. Shuangling Liu
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  3. Jinming Wang
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  4. Jianhai Zhang
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  6. Jundong Wang
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Correspondence to Zilong Sun or Jundong Wang.

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Ruiyan Niu and Shuangling Liu contributed equally to this work.

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Niu, R., Liu, S., Wang, J. et al. Proteomic Analysis of Hippocampus in Offspring Male Mice Exposed to Fluoride and Lead. Biol Trace Elem Res 162, 227–233 (2014). https://doi.org/10.1007/s12011-014-0117-2

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