Abstract
The aim of this study was to investigate microstructure and ultrastructure alterations in the pallium of immature mice exposed to cadmium. Forty immature mice were randomly divided into control, 1/100 LD50 (1.87 mg/kg, low), 1/50 LD50 (3.74 mg/kg, medium), and 1/25 LD50 (7.48 mg/kg, high) dose groups. After oral cadmium exposure for 40 days, the pallium of mice was obtained for microstructure and ultrastructure studies. The results showed that both microstructure and ultrastructure alterations of the pallium were observed in all treated mice and the most obvious alterations were in the high dose group. Microstructural analysis showed seriously congested capillary in the pia mater of the pallium in the high cadmium group. Meanwhile, vacuolar degenerate or karyopyknosis presented in some neurocytes, capillary quantity, and the number of apoptotic cells increased, some neurocytes became hypertrophy, the pia mater separated from the cortex, and local hemorrhage and accompanied inflammatory cell infiltration were also observed. Ultrastructural analysis showed that rough endoplasmic reticulum was expanded, heterochromatin marginalized, perinuclear space distinctly broadened, swelling and vacuolization mitochondria appeared, synapse was swelling, presynaptic and postsynaptic membranes presented fusion, and most of mitochondrial cristae were ambiguous. The results indicated that cadmium exposure for 40 days induced dose-dependent microstructure and ultrastructure alterations in pallium of immature mice.
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All procedures performed in studies involving animals were in accordance with the ethical standards of the institution at which the studies were conducted.
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This study was supported by the Grant of Aid Project for Leading Young Teachers in Henan Provincial Institutions of Higher Education of China (Grant No.2010GGJS-136) and Science and Technology Research Important Project of Education Department Henan Province (Grant No.13A230142).
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The authors declare that they no conflict of interest.
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X. F. Yang and Q. G. Han contributed equally to this work.
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Yang, X.F., Han, Q.G., Liu, D.Y. et al. Microstructure and Ultrastructure Alterations in the Pallium of Immature Mice Exposed to Cadmium. Biol Trace Elem Res 174, 105–111 (2016). https://doi.org/10.1007/s12011-016-0657-8
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DOI: https://doi.org/10.1007/s12011-016-0657-8