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Effects of Fluoride on DNA Damage and Caspase-Mediated Apoptosis in the Liver of Rats

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Abstract

Fluoride compounds are abundant and widely distributed in the environment at a variety of concentrations. Further, fluoride induces toxic effects in target organs such as the liver. In this study, we investigated liver histopathology, DNA damage, apoptosis, and the mRNA and protein expressions of caspase-3 and −9 in the rat livers by administering varying concentrations of fluoride (0, 50, 100, 200 mg/L ) for 120 days. The results showed fluoride-induced morphological changes and significantly increased apoptosis and DNA damage in rats exposed to fluoride, especially in response to higher doses. The immunohistochemical and qRT-PCR results indicated that caspase-3, caspase-9 protein positive expression and mRNA relative expression enhanced with increasing NaF concentration. In summary, our findings suggest that chronic exposure to fluoride causes damages to liver histopathology and leads to liver apoptosis through caspase-mediated pathways.

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Acknowledgments

This work was financed by grants from the China National Natural Science Foundation (No. 31302158), the Key Project of the Department of Medicine and Technology (No. 2011080), the Special Funds for Experimental Animal Technologies in Shanxi Province of China (No. 2012 K02), the Shanxi Province Science and Technology Bureau Program (No. 2011021030–1), and the Youth Fund Projects of Shanxi Medical University (No. 02201429).

Conflicts of Interest

No conflicts of interest, financial or otherwise are declared by the author(s).

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Authors and Affiliations

  1. Laboratory Animal Center, Shanxi Key Laboratory of Experimental Animal Science and Animal Model of Human Disease, Shanxi Medical University, Road Xinjian 56, Taiyuan, 030001, China

    Guo Hua Song, Fu Bing Huang, Ji Ping Gao, Mao Lin Liu, Wen Biao Pang & Xia Yang

  2. Insititute of Radiation Protection of China, Taiyuan, 030006, Shanxi, China

    Wei bin Li

  3. Department of Biology, Taiyuan Normal University, Taiyuan, 030031, China

    Xiao Yan Yan

  4. Shanxi Key Laboratory of Ecological Animal Science and Environmental Medicine, Shanxi Agricultural University, Taigu, 030801, China

    Mei Jun Huo

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  1. Guo Hua Song
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  2. Fu Bing Huang
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  4. Mao Lin Liu
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Correspondence to Guo Hua Song.

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Song, G.H., Huang, F.B., Gao, J.P. et al. Effects of Fluoride on DNA Damage and Caspase-Mediated Apoptosis in the Liver of Rats. Biol Trace Elem Res 166, 173–182 (2015). https://doi.org/10.1007/s12011-015-0265-z

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  • DOI: https://doi.org/10.1007/s12011-015-0265-z

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