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Effects of Dietary Selenium Deficiency on mRNA Levels of Twenty-One Selenoprotein Genes in the Liver of Layer Chicken

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Abstract

Selenium (Se) is an essential trace element in many life forms due to its occurrence as selenocysteine (Sec) residue in selenoproteins. However, little is known about the expression pattern of selenoproteins in the liver of layer chicken. To investigate the effects of Se deficiency on the mRNA expressions of selenoproteins in the liver tissue of layer chickens, 1-day-old layer chickens were randomly allocated into two groups (n = 120/group). The Se-deficient group (−Se) was fed a Se-deficient corn–soy basal diet; the Se-adequate group as control (+Se) was fed the same basal diet supplemented with Se at 0.15 mg/kg (sodium selenite). The liver tissue was collected and examined for mRNA levels of 21 selenoprotein genes at 15, 25, 35, 45, 55, and 65 days old. The data indicated that the mRNA expressions of Gpx1, Gpx2, Gpx3, Gpx4, Sepn1, Sepp1, Selo, Sepx1, Selu, Txnrd1, Txnrd2, Txnrd3, Dio1, Dio2, SPS2, Selm, SelPb, Sep15, and Sels were decreased (p < 0.05), but not the levels of Dio3 and Seli (p > 0.05). The results showed that the mRNA levels of 19 selenoprotein (except Seli and Dio3) genes in the layer chicken liver were regulated by diet Se level. The present study provided some compensated data about the roles of Se in the regulation of selenoproteins.

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Acknowledgments

This work was supported by the Graduate innovative research project in Heilongjiang Province China (No. YJSCX-005HLJ), China Postdoctoral Science Foundation (No. 2012 M520702), And Startup Foundation for Doctors of Northeast Agricultural University, China (No. 2012RCB92).

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Authors and Affiliations

  1. Department of Veterinary Medicine, Northeast Agricultural University, Harbin, 150030, People’s Republic of China

    C. P. Liu, S. L. Lin, X. S. Wang & S. Li

  2. College of Animal Science and Technology, Northeast Agricultural University, Harbin, 150030, People’s Republic of China

    J. Fu

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  1. C. P. Liu
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  2. J. Fu
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Correspondence to S. Li.

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Liu, C.P., Fu, J., Lin, S.L. et al. Effects of Dietary Selenium Deficiency on mRNA Levels of Twenty-One Selenoprotein Genes in the Liver of Layer Chicken. Biol Trace Elem Res 159, 192–198 (2014). https://doi.org/10.1007/s12011-014-0005-9

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  • DOI: https://doi.org/10.1007/s12011-014-0005-9

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