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Selenium Supplementation Modulates Zinc Levels and Antioxidant Values in Blood and Tissues of Diabetic Rats Fed Zinc-Deficient Diet

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Abstract

Diabetes mellitus is associated to a reduction of antioxidant defenses that leads to oxidative stress and complications in diabetic individuals. The present study was undertaken to investigate the effect of selenium on blood biochemical parameters, antioxidant enzyme activities, and tissue zinc levels in alloxan-induced diabetic rats fed a zinc-deficient diet. The rats were divided into two groups; the first group was fed a zinc-sufficient diet, while the second group was fed a zinc-deficient diet. Half of each group was treated orally with 0.5 mg/kg sodium selenite. Tissue and blood samples were taken from all animals after 28 days of treatment. At the end of the experiment, the body weight gain and food intake of the zinc-deficient diabetic animals were lower than that of zinc-adequate diabetic animals. Inadequate dietary zinc intake increased glucose, lipids, triglycerides, urea, and liver lipid peroxidation levels. In contrast, serum protein, reduced glutathione, plasma zinc and tissue levels were decreased. A zinc-deficient diet led also to an increase in serum glutamate oxaloacetate transaminase, glutamate pyruvate transaminase, and liver glutathione-S-transferase and to a decrease in serum alkaline phosphatase activity and glutathione peroxidase. Selenium treatment ameliorated all the values approximately to their normal levels. In conclusion, selenium supplementation presumably acting as an antioxidant led to an improvement of insulin activity, significantly reducing the severity of zinc deficiency in diabetes.

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Acknowledgment

ZK formulated the present hypothesis. ZK, MN, and MF were responsible for writing the report. WF was responsible for analysis of the data. This work was supported by research project no. FOI 120070054 funded by the Ministry of Higher Education, Algeria. There is no conflict interest.

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Authors and Affiliations

  1. Laboratory of Biochemistry and Microbiology Application, Department of Biochemistry, Faculty of Sciences, Badji Mokhtar University, Annaba, Algeria

    Wided Fatmi & Zine Kechrid

  2. Neuroscience Research Center, Suleyman Demirel University, Isparta, Turkey

    Mustafa Nazıroğlu

  3. Department of Chemical and Biochemical Engineering, InstitutoTecnológico de Tijuana, Tijuana, NM, USA

    Manuel Flores-Arce

  4. Medical Faculty, Suleyman Demirel University, Tıp FakültesiDekanlikBinasi, Isparta, TR, 32260, Turkey

    Mustafa Nazıroğlu

Authors
  1. Wided Fatmi
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  2. Zine Kechrid
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  3. Mustafa Nazıroğlu
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  4. Manuel Flores-Arce
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Correspondence to Zine Kechrid or Mustafa Nazıroğlu.

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Fatmi, W., Kechrid, Z., Nazıroğlu, M. et al. Selenium Supplementation Modulates Zinc Levels and Antioxidant Values in Blood and Tissues of Diabetic Rats Fed Zinc-Deficient Diet. Biol Trace Elem Res 152, 243–250 (2013). https://doi.org/10.1007/s12011-013-9613-z

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  • DOI: https://doi.org/10.1007/s12011-013-9613-z

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