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Effects of Dietary Manganese on Cu, Fe, Zn, Ca, Se, IL-1β, and IL-2 Changes of Immune Organs in Cocks

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Abstract

Manganese (Mn) is an essential trace element required for normal development and bodily function. However, little is known about the effect of excessive amounts of Mn in immune organs of poultry. The aim of this study was to investigate the effect of dietary Mn on the content of trace elements, such as copper (Cu), iron (Fe), zinc (Zn), calcium (Ca), and selenium (Se), and the mRNA level of IL-1β and IL-2 in immune organs (spleen, thymus, and bursa of Fabricius) and the content of IL-1β and IL-2 in serum of poultry. Fifty-day-old male Hyline cocks were fed either a commercial diet or a Mn-supplemented diet containing 600, 900, and 1,800 mg/kg. The immune organs were collected at 30, 60, and 90 days, respectively, and the content of trace elements and the mRNA level of IL-1β and IL-2 were examined; the serum were collected and the IL-1β and IL-2 contents detected. The results showed that Mn content in immune organs increased and Fe, Zn, and Ca contents decreased; however, Cu and Se contents showed no difference. IL-1β and IL-2 mRNA levels and IL-1β and IL-2 contents decreased. The present study demonstrates that excess exposure to Mn results in metal accumulations in immune organs. Manganism can disturb the balance of trace elements in immune organs and induce immune suppression in the molecular level; therefore, the immune function of cocks are also suppressed after manganism.

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Acknowledgments

This study was supported by the National Natural Science Foundation of China (no. 30871902).

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Authors and Affiliations

  1. Key Laboratory for Food Science and Engineering, Harbin University of Commerce, Harbin, 150076, People’s Republic of China

    Xiaofei Liu & Chunran Han

  2. College of Veterinary Medicine, Northeast Agricultural University, Harbin, 150030, People’s Republic of China

    Xiaofei Liu, Zhipeng Li, Ziwei Zhang & Shiwen Xu

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  1. Xiaofei Liu
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Correspondence to Shiwen Xu.

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Xiaofei Liu and Zhipeng Li contributed equally to this work.

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Liu, X., Li, Z., Han, C. et al. Effects of Dietary Manganese on Cu, Fe, Zn, Ca, Se, IL-1β, and IL-2 Changes of Immune Organs in Cocks. Biol Trace Elem Res 148, 336–344 (2012). https://doi.org/10.1007/s12011-012-9377-x

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