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Marginal Copper Deficiency Increases Liver Neutrophil Accumulation After Ischemia/Reperfusion in Rats

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Abstract

Copper deficiency can cause a host of major cardiovascular complications including an augmented inflammatory response through effects on both neutrophils and the microvascular endothelium. In the present study, we evaluated the effect of marginal copper deficiency on the neutrophilic response to hepatic ischemia/reperfusion injury, a condition that induces an inflammatory response. Male weanling Sprague–Dawley rats were fed purified diets which were either copper-adequate (6.3 mg/kg) or copper-marginal (1.62 mg/kg) for 4 weeks prior to undergoing 90 min of partial hepatic ischemia followed by 8 h of reperfusion. Liver injury was assessed by serum levels of alanine aminotransferase and by liver histology. Liver neutrophil accumulation was determined by tissue myeloperoxidase content. There was no significant difference in liver injury between copper-adequate and copper-marginal rats. However, liver neutrophil accumulation was significantly increased in copper-marginal rats. These findings were confirmed histologically. Liver expression of the adhesion molecule, intercellular adhesion molecule-1 (ICAM-1), was increased in copper-marginal rats compared to copper-adequate rats. The results suggest that neutrophil accumulation is increased through enhanced ICAM-1 expression in liver of copper-marginal rats after ischemia/reperfusion, but that this does not result in increased liver injury.

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Acknowledgements

We thank Sharon Gordon for her expert technical assistance. This study was supported by National Institute of Diabetes and Digestive and Kidney Diseases Grant DK-55030. The US Department of Agriculture, Agricultural Research Service, Northern Plains Area, is an equal opportunity/affirmative action employer, and all agency services are available without discrimination.

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Authors and Affiliations

  1. The Department of Surgery, University of Cincinnati, Cincinnati, OH, 45267, USA

    Nozomu Sakai, Thomas Shin, Rebecca Schuster, John Blanchard & Alex B. Lentsch

  2. Grand Forks Human Nutrition Research Center, Grand Forks, ND, 58202, USA

    William Thomas Johnson

  3. Department of Physiology and Biophysics, Health Sciences Center A1111, University of Louisville, Louisville, KY, 40292, USA

    Dale A. Schuschke

Authors
  1. Nozomu Sakai
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  2. Thomas Shin
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  3. Rebecca Schuster
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  4. John Blanchard
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  5. Alex B. Lentsch
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  6. William Thomas Johnson
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  7. Dale A. Schuschke
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Correspondence to Dale A. Schuschke.

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Sakai, N., Shin, T., Schuster, R. et al. Marginal Copper Deficiency Increases Liver Neutrophil Accumulation After Ischemia/Reperfusion in Rats. Biol Trace Elem Res 142, 47–54 (2011). https://doi.org/10.1007/s12011-010-8743-9

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  • DOI: https://doi.org/10.1007/s12011-010-8743-9

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