Abstract
A high concentration of vanadium (V) has toxic effects on human and animals and is one of environmental pollutants. In the present study, we have conducted a fundamental study using cultured Vero cells from monkey kidney for the future environmental monitoring. Orthovanadate (VAN), one of V compounds, of 10−10 and 10−8 M did not affect the cell growth although the higher concentration of above 10−6 M VAN inhibited the cell growth accompanied with the decrease in cell numbers and morphological changes. Given that the washing method with ice-cold Li is also effective for determination of the cellular Na content, we used this method for the determination of the V content of the Vero cells. The V distributions in Vero cell; in the 10−3 M VAN solution, extracellular and intracellular were obtained as 1:0.564:0.036 and 1:0.662:0.098 at 60 and 120 min after the treatment of VAN. The intracellular V content was 10% of the applied concentration of VAN. Consequently, it was suggested that V concentration of 10−7 and 10−6 M in the tissue and environment, respectively, might become the threshold concentration; a criterion of the environmental contamination when we carry out environmental monitoring.
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Acknowledgment
This study was funded by grant No. 13660328 in 2001-2003 and grant No. 20580344-0001 (2008) from the Japanese Ministry of Education.
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Mochizuki, M., Kudo, E., Kikuchi, M. et al. A Basic Study on the Biological Monitoring for Vanadium—Effects of Vanadium on Vero Cells and the Evaluation of Intracellular Vanadium Contents. Biol Trace Elem Res 142, 117–126 (2011). https://doi.org/10.1007/s12011-010-8741-y
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DOI: https://doi.org/10.1007/s12011-010-8741-y