Abstract
Oxidative stress is a common mechanism by which chemical toxicity can occur in the liver. The aim of the studies conducted has been to determine what influence the administration of methionine during intoxication with sodium fluoride may have upon the selected enzymes of the antioxidative system in rat liver. The experiment was carried out on Wistar FL rats (adult females) that, for 35 days, were administered distilled water, NaF, or NaF with methionine (doses: 10 mg NaF/kg bw/day, 10 mg Met/kg bw/day). The influence of administered NaF and Met was examined by analyzing the activity of the antioxidative enzymes: superoxide dismutase, catalase, glutathione peroxidase, glutathione reductase, and glutathione transferase in the liver. The results suggest that fluoride reduces the efficiency of the enzymatic antioxidative system in the liver. Administration of methionine during intoxication with sodium fluoride does not have an advantageous influence upon the activity of superoxide dismutase, catalase, reductase, and glutathione transferase in the liver. The slight increase of the activity of glutathione peroxidase after administration of methionine may indicate its protective influence upon that enzyme.
Similar content being viewed by others
References
Wang YN, Xiao KQ, Liu JL, Dallner G, Guan ZZ (2000) Effect of long term fluoride exposure on lipid composition in rat liver. Toxicology 146:161–169
Shashi A, Thapar SP (2000) Histopathology of fluoride-induced hepatotoxicity in rabbits. Fluoride 34:34–42
Shanthakumari D, Srinivasalu S, Subramanian S (2004) Effect of fluoride intoxication on lipid peroxidation and antioxidant status in experimental rats. Toxicology 204:219–228
Birkner E, Grucka-Mamczar E, Machoy Z, Tarnawski R, Polaniak R (2000) Disturbance of protein metabolism in rats after acute poisoning with sodium fluoride. Fluoride 33:182–186
Grucka-Mamczar E, Birkner E, Zalejska-Fiolka J, Machoy Z, Kasperczyk S, Błaszczyk I (2007) Influence of extended exposure to sodium fluoride and caffeine on the activity of carbohydrate metabolism enzymes in rat blood serum and liver. Fluoride 40:62–66
Kappus H (1992) Oxidative stress in chemical toxicity. In: Csomós G, Feher J (eds) Free radicals and the liver. Springer, Berlin, pp 13–20
He LF, Chen JG (2006) DNA damage, apoptosis and cell cycle changes induced by fluoride in rat oral mucosal cells and hepatocytes. World J Gastroenterol 12:1144–1148
Stadtman ER, Moskovitz J, Levine RL (2003) Oxidation of methionine residues of proteins: biological consequences. Antioxid Redox Signal 5:577–582
Błaszczyk I, Grucka-Mamczar E, Kasperczyk S, Birkner E (2009) Influence of methionine upon the concentration of malondialdehyde in the tissue and blood of rats exposed to sodium fluoride. Biol Trace Elem Res 129:229–238
Błaszczyk I, Grucka-Mamczar E, Kasperczyk S, Birkner E (2010) Influence of methionine upon the activity of antioxidative enzymes in the kidney of rats exposed to sodium fluoride. Biol Trace Elem Res 133:60–70
Stadtman ER, Moskovitz J, Berlett BS, Levine RL (2002) Cyclic oxidation and reduction of protein methionine residues is an important antioxidant mechanism. Mol Cell Biochem 234–235:3–9
Abdel-Wahhab MA, Nada SA, Arbid MS (1999) Ochratoxicosis: prevention of developmental toxicity by L-methionine in rats. J Appl Toxicol 19:7–12
Reser D, Rho M, Dewan D, Herbst L, Li G, Stupak H, Zur K, Romaine J, Frenz D, Goldbloom L, Kopke R, Arezzo J, Van De Water T (1999) L- and D-methionine provide long term protection against CDDP-induced ototoxicity in vivo, with partial in vitro and in vivo retention of antineoplastic activity. Neurotoxicology 20:731–748
Oyanagui Y (1984) Revaluation of assay methods and establishment of kid for superoxide dismutase activity. Anal Biochem 142:290–296
Aebi H (1984) Catalase in vitro. Methods Enzymol 105:121–126
Paglia D, Valentine W (1967) Studies on the quantities and qualitative characterization of erythrocyte glutatione peroxidase. J Lab Clin 70:158–169
Richterich R (1971) Clinical chemistry. PZWL, Warsaw
Habig WH, Jakoby WB (1981) Assays for differentiation of glutathione S-transferases. Methods Enzymol 77:398–405
Dröge W (2002) Free radicals in the physiological control of cell function. Physiol Rev 82:47–95
Stadtman ER, Levine RL (2003) Free radical-mediated oxidation of free amino acids and amino acid residues in proteins. Amino Acids 25:207–218
Moskovitz J (2005) Roles of methionine sulfoxide reductases in antioxidant defense, protein regulation and survival. Curr Pharm Des 11:1451–1457
Krechniak J, Inkielewicz I (2005) Correlations between fluoride concentrations and free radical parameters in soft tissues of rats. Fluoride 38:293–296
Zhan X, Wang M, Xu ZR, Li WF, Li JX (2006) Effects of fluoride on hepatic antioxidant system and transcription of Cu/Zn SOD gene in young pigs. J Trace Elem Med Biol 20:83–87
Corrales FJ, Perez-Mato I, Sanchez Del Pino MM, Ruiz F, Castro C, Garcia-Trevijano ER, Latasa U, Martinez-Chantar ML, Martinez-Cruz A, Avila MA, Mato JM (2002) Regulation of mammalian liver methionine adenosytransferase. J Nutr 132:2377–2381
Patra RC, Swarup D, Dwivedi SK (2001) Antioxidant effects of α tocopherol, ascorbic acid and L-methionine on lead induced oxidative stress to the liver, kidney and brain in rats. Toxicology 162:81–88
Patra RC, Swarup D (2004) Effect of antioxidant ascorbic acid, l-methionine or α tocopherol alone or along with chelator on cardiac tissue of lead-treated rats. Veterinarski Arh 74:235–244
Murphy-Chutarian DR, Wexman MP, Grieco AJ, Heiniger JA, Glassman E (1985) Methionine intolerance: a possible risk factor for coronary artery disease. J Am Col Cardiol 6:725–730
Bourdon E, Loreau N, Lagrost L, Blache D (2005) Differential effects of cysteine and methionine residues in the antioxidant activity of human serum albumin. Free Radic Res 39:15–20
Selvam R, Ravichandran V (1993) Restoration of tissue antioxidants and prevention of renal stone deposition in B6 deficient rats fed with vitamin E or methionine. Indian J Exp Biol 31:882–887
Maulik G, Ghosh N, Sengupta T, Chattopadhyay D, Chakraborty AK, Chatterjee GC (1992) Curative effect of methionine on certain enzymes of chick kidney cortex under lanthanum toxicity situation. Indian J Exp Biol 30:1166–1169
Yin J, Chu J, Ricci M, Brems DN, Wang DI, Trout BL (2004) Effects of antioxidants on the hydrogen peroxide-mediated oxidation of methionine residues in granulocyte colony-stimulating factor and human parathyroid hormone fragment 13–34. Pharm Res 21:2377–2383
Wąsowicz W, Gołębiowska M, Chlebna-Sokół D (1988) Increased urinary excretion of selenium in children—a response to surplus fluoride in drinking water. Trace Elem Med 5:43–46
Yu Q, Cerklewski FL, Whanger PD, Hedstrom O, Ridlington JW (1992) Effect of dietary fluoride on selenite toxicity in rat. Biol Trace Elem Res 34:265–278
Park YC, Whanger PD (1995) Toxicity, metabolism and absorption of selenite by isolated rat hepatocytes. Toxicology 100:151–162
Peterson FJ, Lindemann NJ, Duquette PH, Holtzman JL (1992) Potentiation of acute acetaminophen lethality by selenium and vitamin deficiency in mice. J Nutr 122:74–81
Beatty PW, Reed DJ (1980) Involvement of the cystathionine pathway in the biosynthesis of glutathione by isolated rat hepatocytes. Arch Biochem Biophys 204:80–87
Shoveller AK, Stoll B, Ball RO, Burrin DG (2005) Nutritional and functional importance of intestinal sulfur amino acid metabolism. J Nutr 135:1609–1612
Garcia RAG, Stipanuk MH (1992) The splanchnic organs, liver and kidney have unique roles in the metabolism of sulfur amino acids and their metabolites in rats. J Nutr 122:1693–1701
Yur F, Belge F, Mert N, Yörük I (2003) Changes in erythrocyte parameters on fluorotic sheep. Fluoride 36:152–156
Sokołowska-Jeżewicz M, Kryczyk A, Dudzik P, Włodek L (2007) Glutatione transferases—structure and functions. β-lyase-dependent bioactivation of cysteine S-conjugates. Postepy Biochem 53:374–388
Erdmann K, Grosser N, Schröder H (2005) L-Methionine reduces oxidant stress in endothelial cells: role of heme oxygenase-1, ferritin, and nitric oxide. AAPS Journal 7:195–200
Slyshenkov VS, Slevalye AA, Liopo AV, Wojtczak L (2002) Protective role of l-methionine against free radical damage of rat brain synaptosomes. Acta Biochim Pol 49:907–916
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Błaszczyk, I., Birkner, E. & Kasperczyk, S. Influence of Methionine on Toxicity of Fluoride in the Liver of Rats. Biol Trace Elem Res 139, 325–331 (2011). https://doi.org/10.1007/s12011-010-8664-7
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s12011-010-8664-7