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Screening and Identification of a Strain with Protease and Phytase Activities and Its Application in Soybean Meal Fermentation

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Abstract

The aims of the study were to degrade the anti-nutritional factors (ANFs) such as phytic acid, glycinin, and β-conglycinin and improve the values of soybean meal (SBM). Firstly, in this study, a strain PY-4B which exhibited the best enzymatic activities of protease (403.3 ± 17.8 U/mL) and phytase (62.9 ± 2.9 U/mL) was isolated and screened among the isolates. Based on the analysis of physiological and biochemical characteristics and 16S rDNA sequence, the strain PY-4B was identified and named as Pseudomonas PY-4B. Next, Pseudomonas PY-4B was applied to fermentation of SBM. The results showed that the contents of glycinin and β-conglycinin were decreased by 57–63%, and the phytic acid was remarkably degraded by 62.5% due to the fermentation of SBM by Pseudomonas PY-4B. The degradation of glycinin and β-conglycinin resulted in increase of contents of water-soluble proteins and amino acids in fermented SBM. Moreover, Pseudomonas PY-4B exhibited no hemolytic activity and slight inhibitory effect on the growth of pathogen Staphylococcus aureus and the wide range of pH tolerance (3 to 9). In summary, our study indicates that isolated strain Pseudomonas PY-4B is a safe and applicable strain and has the ability to effectively degrade the ANFs (phytic acid, glycinin, and β-conglycinin) in SBM by fermentation.

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Data Availability

The datasets generated during and/or analyzed during the current study are available from the corresponding author on reasonable request.

Abbreviations

AA:

Amino acids

ANFs:

Anti-nutritional factors

FSBM:

Fermented soybean meal

PSM:

Phytase screening media

SBM:

Soybean meal

SD:

Standard deviation

SDS-PAGE:

Sodium dodecyl sulfate–polyacrylamide gel electrophoresis

SEM:

Scanning electron microscope

SMKP:

Skimmed milk powder solid medium

TMB:

3,3′,5,5′-Tetramethylbenzidine

VP:

Voges-Proskauer

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Funding

This work was supported by Key Research and Development Program of Zhejiang Province (2021C04016) and Pioneer and Leading Goose R&D Program of Zhejiang (2022C04020).

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Authors and Affiliations

  1. Zhejiang Provincial Key Laboratory of Biometrology and Inspection & Quarantine, College of Life Sciences, China Jiliang University, Xueyuan Street, Xiasha Higher Education District, Hangzhou, Zhejiang Province, 310018, People’s Republic of China

    Hengyi Lin, Zheng Ma & Xiaoping Yu

  2. Department of Aquaculture, Zhejiang Ocean University, Zhoushan, 316000, Zhejiang Province, China

    Tao Han & Jiteng Wang

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  1. Hengyi Lin
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  2. Tao Han
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  3. Jiteng Wang
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  4. Zheng Ma
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Contributions

H.Y.L. conducted experiments. H.Y.L. and Z.M. wrote the original draft. T.H. and J.T.W. designed the experiments. Z.M. revised this article. X.Y.Y. polished English and checked the final version. All authors have read and approved the final manuscript and, therefore, have full access to all the data in the study and take responsibility for the integrity and security of the data.

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Correspondence to Zheng Ma.

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Lin, H., Han, T., Wang, J. et al. Screening and Identification of a Strain with Protease and Phytase Activities and Its Application in Soybean Meal Fermentation. Appl Biochem Biotechnol 196, 790–803 (2024). https://doi.org/10.1007/s12010-023-04568-w

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