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Genetic and Phenotypic Characteristics of a Multi-strain Probiotic for Broilers

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Abstract

Bacteria isolated from different segments of the gastro-intestinal tract (GIT) of healthy free-range broilers were screened for probiotic properties. Six strains were selected and identified as Lactobacillus gallinarum, Lactobacillus johnsonii, Lactobacillus salivarius, Lactobacillus crispatus, Enterococcus faecalis and Bacillus amyloliquefaciens based on 16S rRNA, gyrB and recA gene sequence analyses. All six strains produced exopolysaccharides (EPS) and formed biofilms under conditions simulating the broiler GIT. Lactobacillus johnsonii DPN184 and L. salivarius DPN181 produced hydrogen peroxide, and L. crispatus DPN167 and E. faecalis DPN94 produced bile salt hydrolase (BSH) and phytase. Bacillus amyloliquefaciens DPN123 produced phytase, amylase, surfactin and iturin A1. No abnormalities were observed when broilers were fed the multi-strain combination, suggesting that it could be used as a probiotic.

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  • 02 April 2020

    The authors would like to correct the errors in the publication of the original article.

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  1. Department of Microbiology, University of Stellenbosch, Matieland, Stellenbosch, 7602, South Africa

    Deon P. Neveling, Jayesh J. Ahire & Leon M. T. Dicks

  2. Department of Biochemistry, Stellenbosch University, Stellenbosch, South Africa

    Wikus Laubscher & Marina Rautenbach

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Neveling, D.P., Ahire, J.J., Laubscher, W. et al. Genetic and Phenotypic Characteristics of a Multi-strain Probiotic for Broilers. Curr Microbiol 77, 369–387 (2020). https://doi.org/10.1007/s00284-019-01797-3

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