Abstract
Microbially induced calcium carbonate precipitation (MICP) is an immensely growing technique that utilizes the metabolic pathways of bacteria to form calcite precipitation throughout the soil matrix, leading to improve geotechnical engineering properties. However, the excessive number of treatments limited the application of MICP for strengthening calcareous sand. To reduce the number of treatments and develop efficiencies, this paper investigates the optimized treatment protocol of adding aluminum ion flocculants to the cementing solution to accelerate the curing rate of the MICP and its effect. The results show that adding a certain concentration of AlCl3 to the cementing solution can resulted in a rapid increase in strength of the calcareous sand column. When 0.02 M aluminum chloride was added to the cementing solution, the unconfined compressive strength of the sand column reached 827 kPa after three treatments, and it reached 2 MPa after five treatments, while the control group needed to be treated 10 and 15 times, respectively, to reach equivalent strengths. In this paper, the unconfined compressive strength of the sand column formed using the proposed method was 27–40 times that of the control group at the same calcium carbonate content. The presented experimental approach can be used as a tool to design the treatment protocol for the engineering application of MICP-reinforced calcareous sand in practice.
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All data included in this study are available upon request by contact with the corresponding author.
References
Kang, C. H., Choi, J. H., Noh, J., Kwak, D. Y., Han, S. H., & So, J. S. (2014). Microbially induced calcite precipitation-based sequestration of strontium by sporosarcina pasteurii WJ-2. Applied Biochemistry and Biotechnology, 174, 2482–2491.
Achal, V., & Pan, X. (2014). Influence of calcium sources on microbially induced calcium carbonate precipitation by Bacillus sp CR2. Applied Biochemistry and Biotechnology, 173, 307–317.
Mujah, D., Shahin, M. A., & Cheng, L. (2017). State-of-the-art review of biocementation by Microbially Induced Calcite Precipitation (MICP) for soil stabilization. Geomicrobiology Journal, 34, 524–537.
Song, C., Elsworth, D., Zhi, S., & Wang, C. (2021). The influence of particle morphology on microbially induced CaCO3 clogging in granular media. Marine Georesources & Geotechnology, 39, 74–81.
Fang, X., Shen, C., Chu, J., Wu, S., & Li, Y. (2015). An experimental study of coral sand enhanced through microbially-induced precipitation of calcium carbonate. Rock and Soil Mechanics, 36, 2773–2779.
Harkes, M. P., van Paassen, L. A., Booster, J. L., Whiffin, V. S., & van Loosdrecht, M. C. M. (2010). Fixation and distribution of bacterial activity in sand to induce carbonate precipitation for ground reinforcement. Ecological Engineering, 36, 112–117.
Bains, A., Dhami, N. K., Mukherjee, A., & Reddy, M. S. (2015). Influence of exopolymeric materials on bacterially induced mineralization of carbonates. Applied Biochemistry and Biotechnology, 175, 3531–3541.
Sarayu, K., Iyer, N. R., & Murthy, A. R. (2014). Exploration on the biotechnological aspect of the ureolytic bacteria for the production of the cementitious materials-a review. Applied Biochemistry and Biotechnology, 172, 2308–2323.
Anbu, P., Kang, C. H., Shin, Y. J., & So, J. S. (2016). Formations of calcium carbonate minerals by bacteria and its multiple applications. Springerplus, 5, 250.
Hammes, F., & Verstraete*, W. (2002). Key roles of pH and calcium metabolism in microbial carbonate precipitation. Reviews in Environmental Science & Biotechnology, 1, 3–7.
Mortensen, B. M., Haber, M. J., DeJong, J. T., Caslake, L. F., & Nelson, D. C. (2011). Effects of environmental factors on microbial induced calcium carbonate precipitation. Journal of Applied Microbiology, 111, 338–349.
Al Qabany, A., Soga, K., & Santamarina, C. (2012). Factors affecting efficiency of microbially induced calcite precipitation. Journal of Geotechnical and Geoenvironmental Engineering, 138, 992–1001.
Gorospe, C. M., Han, S. H., Kim, S. G., Park, J. Y., Kang, C. H., Jeong, J. H., & So, J. S. (2013). Effects of different calcium salts on calcium carbonate crystal formation by Sporosarcina pasteurii KCTC 3558. Biotechnology and Bioprocess Engineering, 18, 903–908.
Stocks-Fischer, S., Galinat, J. K., & Bang, S. S. (1999). Microbiological precipitation of CaCO3. Soil Biology & Biochemistry, 31, 1563–1571.
Mitchell, A. C., & Ferris, F. G. (2005). The coprecipitation of Sr into calcite precipitates induced by bacterial ureolysis in artificial groundwater: Temperature and kinetic dependence. Geochimica Et Cosmochimica Acta, 69, 4199–4210.
Okwadha, G. D. O., & Li, J. (2010). Optimum conditions for microbial carbonate precipitation. Chemosphere, 81, 1143–1148.
De Muynck, W., De Belie, N., & Verstraete, W. (2010). Microbial carbonate precipitation in construction materials: a review. Ecological Engineering, 36, 118–136.
Cunningham, A. B., Class, H., Ebigbo, A., Gerlach, R., Phillips, A. J., & Hommel, J. (2019). Field-scale modeling of microbially induced calcite precipitation. Computers & Geosciences, 23, 399–414.
Li, H., Tang, C., Liu, B., Lu, C., Cheng, Q., & Shi, B. (2020). Mechanical behavior of MICP-cemented calcareous sand in simulated seawater environment. Chinese Journal of Geotechnical Engineering, 42, 1931–1939.
Zheng, J., Wu, C., Song, Y., & Cui, M. (2020). Study of the strength test and strength dispersion of MICP-treated calcareous sand. Journal of Harbin Engineering University, 41, 250–256.
Achal, V., Pan, X., & Zhang, D. (2012). Bioremediation of strontium (Sr) contaminated aquifer quartz sand based on carbonate precipitation induced by Sr resistant Halomonas sp. Chemosphere, 89, 764–768.
Cheshomi, A., Mansouri, S., & Amoozegar, M. A. (2018). Improving the shear strength of quartz sand using the microbial method. Geomicrobiology Journal, 35, 749–756.
Martinez, A., Huang, L., & Gomez, M. G. (2019). Thermal conductivity of MICP-treated sands at varying degrees of saturation. Geotechnique Letters, 9, 15–21.
Xiao, Y., Zhao, C., Sun, Y., Wang, S., Wu, H., Chen, H., & Liu, H. (2021). Compression behavior of MICP-treated sand with various gradations. Acta Geotechnica, 16, 1391–1400.
Jafarian, Y., & Javdanian, H. (2020). Dynamic properties of calcareous sand from the Persian Gulf in comparison with siliceous sands database. International Journal of Civil Engineering, 18, 245–249.
Liu, L., Yao, X., Ji, Z., Gao, H., Wang, Z., & Shen, Z. (2021). Cyclic behavior of calcareous sand from the South China Sea. Journal of Marine Science and Engineering, 9(9), 1014.
Xinzhi, W., Ren, W., Qinshan, M., & Xiaopeng, L. I. U. (2009). Study of plate load test of calcareous sand. Rock and Soil Mechanics, 30, 147–151156.
Zhu, C., Chen, H., Meng, Q., & Wang, R. (2014). Microscopic characterization of intra-pore structures of calcareous sands. Rock and Soil Mechanics, 35, 1831–1836.
Kuang, D., Long, Z., Guo, R., & Yu, P. (2021). Experimental and numerical investigation on size effect on crushing behaviors of single calcareous sand particles. Marine Georesources & Geotechnology, 39, 543–553.
Lv, Y., Li, X., Fan, C., & Su, Y. (2021). Effects of internal pores on the mechanical properties of marine calcareous sand particles. Acta Geotechnica, 16, 3209–3228.
Coop, M. R., Sorensen, K. K., Freitas, T. B., & Georgoutsos, G. (2004). Particle breakage during shearing of a carbonate sand. Geotechnique, 54, 157–163.
Xiao, Y., Liu, H., Xiao, P., & Xiang, J. (2016). Fractal crushing of carbonate sands under impact loading. Geotechnique Letters, 6, 199–204.
Xu, L. J., Wang, X., Wang, R., Zhu, C., & Liu, X. (2022). Physical and mechanical properties of calcareous soils: a review. Marine Georesources & Geotechnology, 40, 751–766.
DeJong, J. T., Mortensen, B. M., Martinez, B. C., & Nelson, D. C. (2010). Bio-mediated soil improvement. Ecological Engineering, 36, 197–210.
Lee, M. L., Ng, W. S., & Tanaka, Y. (2013). Stress-deformation and compressibility responses of bio-mediated residual soils. Ecological Engineering, 60, 142–149.
Chu, J., Stabnikov, V., & Ivanov, V. (2012). Microbially induced calcium carbonate precipitation on surface or in the bulk of soil. Geomicrobiology Journal, 29, 544–549.
DeJong, J. T., Fritzges, M. B., & Nusslein, K. (2006). Microbially induced cementation to control sand response to undrained shear. Journal of Geotechnical and Geoenvironmental Engineering, 132, 1381–1392.
Ramachandran, S. K., Ramakrishnan, V., & Bang, S. S. (2001). Remediation of concrete using micro-organisms. Aci Materials Journal, 98, 3–9.
Whiffin, V. S. (2004). Microbial CaCO3 precipitation for the production of biocement. Murdoch University.
Pernitsky, D. J., & Edzwald, J. K. (2006). Selection of alum and polyaluminum coagulants: principles and applications. Journal of Water Supply Research and Technology-Aqua, 55, 121–141.
Verma, A. K., Dash, R. R., & Bhunia, P. (2012). A review on chemical coagulation/flocculation technologies for removal of colour from textile wastewaters. Journal of Environmental Management, 93, 154–168.
Zheng, S., Pang, X., Liu, H., Wang, B., & Zhao, L. (2002). Application of pseudoehmite being synthesized by aluminum sulfate in the preparation of FCC catalyst. Chemical Industry and Engineering Progress, 21, 331–333.
Ishizaka, T., Kobayashi, Y., & Kurokawa, Y. (2003). Alumina coating on quartz glass and nickel substrates using aqueous sol derived from AlCl3 center dot 6H(2)o. Journal Materials Science, 38, 1239–1242.
Choi, S. G., Park, S. S., Wu, S., & Chu, J. (2017). Methods for calcium carbonate content measurement of biocemented soils. Journal of Materials in Civil Engineering, 29(11), 6017015.
Cheng, L., Shahin, M. A., & Chu, J. (2018). Soil bio-cementation using a new one-phase low-pH injection method. Acta Geotechnica, 14, 615–626.
Duan, Y., Xu, G., Yang, D., & Yan, Y. (2019). Influencing factors of calcium ion utilization in MICP mineralized products and analysis of microscopic image. Chemical Industry and Engineering Progress, 38, 2306–2313.
Yin, L., Tang, C., Xie, Y., Lu, C., Jiang, N., & Shi, B. (2019). Factors affecting improvement in engineering properties of geomaterials by microbial-induced calcite precipitation. Rock and Soil Mechanics, 40, 2525–2546.
Duan, J. M., & Gregory, J. (2003). Coagulation by hydrolysing metal salts. Advances in Colloid and Interface Science, 100, 475–502.
Kawasaki, N., Ogata, F., & Tominaga, H. (2010). Selective adsorption behavior of phosphate onto aluminum hydroxide gel. Journal of Hazardous Materials, 181, 574–579.
Achal, V., Pan, X., Fu, Q., & Zhang, D. (2012). Biomineralization based remediation of as(III) contaminated soil by Sporosarcina ginsengisoli. Journal of Hazardous Materials, 201, 178–184.
Funding
This research was funded by the National Natural Science Foundation of China (51578214) & Transformation Program of Scientific and Technological Achievements of Jiangsu Province, China (2021QD07). The authors are very grateful for their financial support.
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Renjie Wei: Methodology, Investigation, Data curation, Writing original draft, Review and Editing. Jie Peng: Conceptualization, Methodology, Supervision, Project administration and Funding acquisition. Liangliang Li: Data curation and Experiment assistant. Zhao Jiang: Experiment assistant. Jiahui Tang: Experiment assistant.
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Wei, R., Peng, J., Li, L. et al. Accelerated Reinforcement of Calcareous sand via Biomineralization with Aluminum Ion Flocculant. Appl Biochem Biotechnol 195, 7197–7213 (2023). https://doi.org/10.1007/s12010-023-04429-6
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DOI: https://doi.org/10.1007/s12010-023-04429-6