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An Investigation of Pepsin Hydrolysate of Short Antibacterial Peptides Derived from Limnospira Sp.

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Abstract

The antimicrobial peptides derived from microalgae have attracted a huge attention due to the insufficient availability of effective drugs from the natural resources. In this study, the enzymatic hydrolysate of protein derived from Limnospira maxima was prepared using pepsin under optimized conditions. The peptides with range of 10 kDa were isolated and purified using the Ultra membrane filtration, SDS-PAGE, and TLC. Furthermore, the peptide sequence was identified and characterized by MALDI-TOF mass spectrometry in which an algal peptide, KLENCNYAVELGK showed a strong signal at 466.68 m/z among seven peptides derived from the pepsin hydrolysate. The FT-IR spectroscopic study confirmed the presence of a characteristic functional group of amino acids in the sequence. The algal derived peptide showed antibacterial properties against Escherichia coli (27 ± 0.5 mm) and Staphylococcus aureus (14 mm ± 0.5). This study paves a way to explore the antibacterial peptide from a novel species, L. maxima (MZ26519) evident to utilize for the novel drug to overcome the conventional approach.

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Funding

This study was supported by a grant from the Incheon National University in 2018.

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Author notes

  1. Sathya R. and MubarakAli D. contributed equally to this work.

Authors and Affiliations

  1. School of Life Sciences, B.S. Abdur Rahman Crescent Institute of Science and Technology, Chennai, 600048, Tamil Nadu, India

    Sathya R., MubarakAli D. & Mehboob Nousheen M.G.

  2. Division of Bioengineering, Incheon National University, Incheon, Republic of Korea

    MubarakAli D. & Jung-Wan Kim

  3. Department of Chemistry, B.S. Abdur Rahman Crescent Institute of Science and Technology, Chennai, 600048, Tamil Nadu, India

    Vasimalai N.

  4. National Repository for Microalgae and Cyanobacteria-Freshwater, Department of Microbiology, Bharathidasan University, Tiruchirappalli, 620024, India

    Thajuddin N.

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  1. Sathya R.
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Contributions

SR: data acquisition and process; MD: review, guidance and proofreading, VN: data processing, guidance, MNM: data acquisition and process, NT: molecular characterization, KJW: review, guidance and proofreading.

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Correspondence to MubarakAli D. or Jung-Wan Kim.

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R., S., D., M., M.G., M.N. et al. An Investigation of Pepsin Hydrolysate of Short Antibacterial Peptides Derived from Limnospira Sp.. Appl Biochem Biotechnol 194, 5580–5593 (2022). https://doi.org/10.1007/s12010-022-04023-2

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