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Purification and identification of novel angiotensin-I converting enzyme (ACE) inhibitory peptides from cultured marine microalgae (Nannochloropsis oculata) protein hydrolysate

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Abstract

Isolation of bioactive compounds and commercialization of marine microalgae sources are interesting targets in future marine biotechnology. Cultured biomass of the marine microalga, Nannochloropsis oculata, was used to purify angiotensin-I converting enzyme (ACE) inhibitory peptides using proteases including pepsin, trypsin, α-chymotrypsin, papain, alcalase, and neutrase. The pepsin hydrolysate exhibited the highest ACE inhibitory activity, compared to the other hydrolysates and then was separated into three fractions (F1, F2, and F3) using Sephadex G-25 gel filtration column chromatography. First fraction (F1) showed the highest ACE inhibitory activity and it was further purified into two fractions (F1-1 and F1-2) using reverse-phase high-performance liquid chromatography. The IC50 value of purified ACE inhibitory peptides were 123 and 173 μM and identified as novel peptides, Gly-Met-Asn-Asn-Leu-Thr-Pro (GMNNLTP; MW, 728 Da) and Leu-Glu-Gln (LEQ; MW, 369 Da), respectively. In addition, nitric oxide production level (%) was significantly increased by the purified peptide (Gly-Met-Asn-Asn-Leu-Thr-Pro) compared to the purified peptide (Leu-Glu-Gln) and other treated pepsin hydrolysate fractions on human umbilical vein endothelial cells (HUVECs). Cell viability assay showed no cytotoxicity on HUVECs with the treated purified peptides and fractions. These results suggest that the isolated peptides from cultured marine microalga, N. oculata protein sources may have potentiality to use commercially as ACE inhibitory agents in functional food industry.

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Acknowledgments

This research was supported by a grant (T32607) from the Korea Basic Science Institute (KBSI).

Conflict of interest

The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. Department of Marine Life Science, Jeju National University, Jeju, 690-756, Republic of Korea

    Kalpa Wishvajith Samarakoon, Ju-Young Ko, Ji-Hyeok Lee, Min-Cheol Kang & You-Jin Jeon

  2. Marine Biology Center for Research and Education, Gangneung-Wonju National University, Gangwon-do, Republic of Korea

    Kwon O-Nam

  3. Jeju Centre, Korea Basic Science Institute (KBSI), Jeju, 690-140, Republic of Korea

    Daekyung Kim

  4. Department of Earth and Marine Sciences, Jeju National University, Jeju, 690-756, Republic of Korea

    Joon Baek Lee

  5. Division of Industry-Academic cooperation, Jeju National University, Jeju, 690-756, Republic of Korea

    Jung-Suck Lee

  6. Marine and Environmental Research Institute, Jeju National University, Jeju, 695-814, Republic of Korea

    You-Jin Jeon

  7. School of Marine Biomedical Sciences, Jeju National University, Jeju, 690-756, Republic of Korea

    You-Jin Jeon

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  1. Kalpa Wishvajith Samarakoon
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  2. Kwon O-Nam
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Correspondence to You-Jin Jeon.

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Samarakoon, K.W., O-Nam, K., Ko, JY. et al. Purification and identification of novel angiotensin-I converting enzyme (ACE) inhibitory peptides from cultured marine microalgae (Nannochloropsis oculata) protein hydrolysate. J Appl Phycol 25, 1595–1606 (2013). https://doi.org/10.1007/s10811-013-9994-6

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  • DOI: https://doi.org/10.1007/s10811-013-9994-6

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