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Nano Hydroxyapatite for Biomedical Applications Derived from Chemical and Natural Sources by Simple Precipitation Method

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Abstract

In the past, bone fractures due to accidents were rectified by surgery and reconstruction of bone structure. In recent times, researchers have been made to find a solution by producing alternate biomaterials. Hydroxyapatite (HAp) is one of the most important bioactive materials used as a substitute for human hard tissue because of its composition being very similar to human bones and teeth. A study has proved that HAp has been used for bone regeneration in clinical trials in the mid-1980. HAp has been used as implant coatings and graft materials and also used as granules, cement, and pastes for bone regenerative applications. HAp coatings on bioimplants improved biocompatibility, bioactivity, and biological fixation. Moreover, some of the deposition methods can be employed to increase the cellular responses of bone regeneration such as sputtering, spraying, electrodeposition, and pulsed layer deposition. The researcher has prepared hydroxyapatite from chemical and natural sources. The surface area and intrinsic properties of the HAp play a vital role in bone-related applications. This can be achieved by synthesizing the HAp from natural sources rather than synthetic materials. The HAp obtained from the chemical source is not fulfilling the requirements of the natural bone. A variety of biowaste materials such as eggshell, crab shell, snail shell, bovine bone, fishbone, and fish scales are available in nature and can be converted to useful calcium source for HAp. The present study is to produce the HAp from biowaste materials like eggshell and chemical sources using the wet precipitation method. The synthesized HAp is coated on the Ti6Al4V alloy using the electrodeposition method, and it is immersed in SBF solution at 37 °C for corrosion testing. The coated samples are investigated by Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), electrochemical study, field emission scanning electron icroscopy (FESEM), energy dispersive X-ray analysis (EDAX), AFM, and antibacterial activity with two different microorganisms. FTIR and XRD confirm the functional groups and crystallinity of the HAp. The good antibacterial activity of the HAp is observed against two bacterial strains. The corrosion studies reveal that the HAp derived from a natural source is eco-friendly and nontoxic and has excellent corrosion resistivity and cell adhesion properties. A strong bond is formed between the naturally derived HAp with bone tissue which is involved in the bio-resorption process and does not pose any side effect to the human body compared to synthetically derived HAp. In addition, the biowaste materials are converted to useful biomaterials and can reduce environmental pollution.

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Authors and Affiliations

  1. Department of Chemistry, Aarupadai Veedu Institute of Technology, Vinayaka Mission’s Research Foundation (Deemed to be University), Rajiv Gandhi Salai, Paiyanoor, Kancheepuram District, 603104, Tamil Nadu, India

    M. Kalpana & R. Nagalakshmi

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  1. M. Kalpana
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  2. R. Nagalakshmi
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Contributions

All authors contributed to the study conception and design. Material preparation and data collection were performed by M. Kalpana. The analysis and interpretation of the data were performed by R. Nagalakshmi. The first draft of the manuscript was written by R. Nagalakshmi, and all authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.

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Correspondence to R. Nagalakshmi.

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Kalpana, M., Nagalakshmi, R. Nano Hydroxyapatite for Biomedical Applications Derived from Chemical and Natural Sources by Simple Precipitation Method. Appl Biochem Biotechnol 195, 3994–4010 (2023). https://doi.org/10.1007/s12010-022-03968-8

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