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Antioxidant, Antibacterial, and Anti-diabetic Activity of Green Synthesized Copper Nanoparticles of Cocculus hirsutus (Menispermaceae)

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Abstract

The emergence of new technologies has led to the discovery of the biological properties of nanoparticles through green approach. In the present investigation, we report the potential antibacterial, antioxidant, and anti-diabetic properties of copper nanoparticle (CuNPs) synthesized by reducing 3 mM copper acetate solution with aqueous leaf extract of Cocculus hirsutus. A colour change from deep brown to dark greenish brown indicated the formation of copper nanoparticles. The so-formed CuNPs were characterized by employing UV spectroscopy, FTIR, SEM, and EDX analyses which described sheet-like structure morphology having typical size of 63.46 nm. Later, the synthesized CuNPs efficiency was evaluated against bacterial pathogens, and was found highly toxic to B. subtilis and S. aureus strains. The synthesized CuNPs were examined through H2O2 and PMA assays which demonstrated the highest free radical scavenging activity. Besides, the resulted CuNPs revealed the higher anti-diabetic efficacy in both the \(\alpha\)-amylase and \(\alpha\) -glucosidase inhibition assays (64.5% ± 0.11 and 68.5% ± 0.11, respectively). Finally, our findings report that C. hirsutus can be exploited as a source for green synthesis of CuNPs, having potent in vitro antioxidant, antibacterial, and anti-diabetic properties.

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Acknowledgements

This research article is from the collaboration between Yogi Vemana University Kadapa, Qassim University, and Universiti Malaysia Kelantan. Many thanks to laboratory assistant that involved in this project.

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Authors and Affiliations

  1. Department Biotechnology and Bioinformatics, Yogi Vemana University, Kadapa, Andhra Pradesh, India, 516005

    Shaik Ameena, Nambi Rajesh, Syeda M. Anjum & Khateef Riazunnisa

  2. Department of Medical Laboratories, College of Applied Medical Sciences, Qassim University, Buraydah, Qassim, 51452, Kingdom of Saudi Arabia

    Habeeb Khadri

  3. Department of Agriculture Science, Faculty of Agro-Based Industry, Universiti Malaysia Kelantan, 17600, Jeli, Kelantan, Malaysia

    Arifullah Mohammed & Zulhisyam Abdul Kari

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  1. Shaik Ameena
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Contributions

Conceptualization, K.R. and S.A.; Formal analysis, S.A.; Methodology, S.A., N.R., H.K., and K.R.; Supervision, K.R.; Validation, K.R., N.R., and S.A.; Writing, N.R., S.A.M., and A.M.; writing—review and editing, K.R., H.K., S.A.M., A.M., and Z.A.K. All authors have read and agreed to the published version of the manuscript.

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Correspondence to Khateef Riazunnisa or Arifullah Mohammed.

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Highlights

CH-CuNPs were synthesized by using C. hirsutus leaf extract.

CH-CuNPs were characterized by employing UV spectroscopy, FTIR, SEM, and EDX.

CH-CuNPs can be exploited as a good source for green synthesis of CuNPs, having potent in vitro antibacterial, antioxidant, and anti-diabetic properties.

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Ameena, S., Rajesh, N., Anjum, S.M. et al. Antioxidant, Antibacterial, and Anti-diabetic Activity of Green Synthesized Copper Nanoparticles of Cocculus hirsutus (Menispermaceae). Appl Biochem Biotechnol 194, 4424–4438 (2022). https://doi.org/10.1007/s12010-022-03899-4

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