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Enhancement of Copper Uptake of Yeast Through Systematic Optimization of Medium and the Cultivation Process of Saccharomyces cerevisiae

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Abstract

Copper is an essential trace element for living organisms. Copper enriched by yeast of Saccharomyces cerevisiae is regarded as the biologically available organic copper supplement with great potentiality for application. However, the lower uptake ratio of copper ions makes the production of copper enriched by yeast uneconomically and environmentally unfriendly. In this study, S. cerevisiae Cu-5 with higher copper tolerance and intracellular copper accumulation was obtained by screening of our yeast strains collection. To increase the uptake ratio of copper ions, the medium composition and cultivation conditions for strain Cu-5 were optimized systematically. A medium comprised of glucose, yeast extract, (NH4)2SO4, and inorganic salts was determined, then a novel cultivation strategy including pH control at 5.5 and increasing amounts of yeast extract for a higher concentration of copper ion in the medium was developed. The uptake ratios of copper ions were more than 90% after combining 50 to 100 mg/L copper ions with 3.5 to 5.0 g/L yeast extract, which is the highest until now and is conducive to the cost-effective and environmentally friendly production of bioactive copper in yeast-enriched form.

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The authors declare that the data and materials are transparent.

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Acknowledgements

The authors would like to acknowledge colleagues from the public technology service center in the Institute of Microbiology, Chinese Academy of Sciences, for their technical assistance. We thank professor Fengyan Bai from the Institute of Microbiology, Chinese Academy of Sciences for providing S. cerevisiae strain S288C.

Funding

This work was financially supported by the National Key R & D Program of China (No. 2018YFD0500605)

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Authors and Affiliations

  1. CAS Key Laboratory of Microbial Physiological and Metabolic Engineering, State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, China

    Xue-Na Guo, Xiao-Xian He, Yan-Fei Cheng, Zhao-Yue Wang & Xiu-Ping He

  2. College of Life Sciences, University of Chinese Academy of Sciences, Beijing, 100049, China

    Xiao-Xian He & Xiu-Ping He

  3. State Key Laboratory of Direct-Fed Microbial Engineering, Beijing DaBeiNong Science and Technology Group Co., Ltd. (DBN), Beijing, 100192, China

    Li-Bin Zhang & Xiu-Mei Bai

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  1. Xue-Na Guo
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Contributions

Xue-Na Guo designed and performed the experiments, processed and interpreted the data, and prepared the manuscript. Xiao-Xian He participated in designing and performing experiments, processing and interpreting data. Li-Bin Zhang, Yan-Fei Cheng, and Xiu-Mei Bai performed the experiments. Zhao-Yue Wang processed and interpreted the data. Xiu-Ping He supervised the experiments and revised the manuscript.

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Correspondence to Xiu-Ping He.

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Xue-Na Guo and Xiao-Xian He contributed equally to this manuscript.

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Guo, XN., He, XX., Zhang, LB. et al. Enhancement of Copper Uptake of Yeast Through Systematic Optimization of Medium and the Cultivation Process of Saccharomyces cerevisiae. Appl Biochem Biotechnol 194, 1857–1870 (2022). https://doi.org/10.1007/s12010-021-03775-7

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