Skip to main content
SpringerLink
Log in

Purification and Identification of Two Antifungal Cyclic Peptides Produced by Bacillus amyloliquefaciens L-H15

  • Published:
Applied Biochemistry and Biotechnology Aims and scope Submit manuscript

Abstract

Bacillus amyloliquefaciens L-H15 with broad spectrum antifungal activity was used as a biocontrol agent to suppress Fusarium oxysporum and other soil-borne fungal plant pathogens. Two antifungal fractions were isolated by bioactivity-guided reversed-phase high-performance liquid chromatography. The two compounds were identified by tandem Q-TOF mass spectroscopy as C15 Iturin A (1) and a novel cyclic peptide with a molecular weight of 852.4 Da (2). Both compounds showed good inhibitory activities against three plant fungal pathogens in cylinder-plate diffusion assay. To our best knowledge, this is the first report on a cyclic antifungal peptide with a molecular weight of 852.4 Da. The strong antifungal activity suggests that the B. amyloliquefaciens L-H15 and its bioactive components might provide an alternative resource for the biocontrol of plant diseases and sustainable agriculture.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6

Similar content being viewed by others

References

  1. Sanjay, R., Ponmurugan, P., & Baby, U. I. (2008). Evaluation of fungicides and biocontrol agents against grey blight disease of tea in the field. Crop Protection, 27, 689–694.

    Article  CAS  Google Scholar 

  2. Gilardi, G., Demarchi, S., Gullino, M. L., & Garibaldi, A. (2015). Management of leaf spot of wild rocket using fungicides, resistance inducers and a biocontrol agent, under greenhouse conditions. Crop Protection, 71, 39–44.

    Article  CAS  Google Scholar 

  3. Cook, R. J. (1993). Making greater use of introduced microorganisms for biological control of plant pathogens. Annual Review of Phytopathology, 31, 53–80.

    Article  CAS  Google Scholar 

  4. Babu, A. N., Jogaia, H., Ito, S., Nagaraj, A. K., & Tran, L. P. (2015). Improvement of growth, fruit weight and early blight disease protection of tomato plants by rhizosphere bacteria is correlated with their beneficial traits and induced biosynthesis of antioxidant peroxidase and polyphenol oxidase. Plant Science, 231, 62–73.

    Article  Google Scholar 

  5. Raza, W., Yuan, J., Ling, N., Huang, Q., & Shen, Q. (2015). Production of volatile organic compounds by an antagonistic strain Paenibacillus polymyxa WR-2 in the presence of root exudates and organic fertilizer and their antifungal activity against Fusarium oxysporum f. sp. niveum. Biological Control, 80, 89–95.

    Article  CAS  Google Scholar 

  6. Jaklitsch, W. M., & Voglmayr, H. (2015). Biodiversity of Trichoderma (Hypocreaceae) in Southern Europe and Macaronesia. Studies in Mycology, 80, 1–87.

    Article  Google Scholar 

  7. Boukaew, S., & Prasertsan, P. (2014). Suppression of rice sheath blight disease using a heat stable culture filtrate from Streptomyces philanthi RM-1-138. Crop Protection, 61, 1–10.

    Article  Google Scholar 

  8. Waweru, B., Turoop, L., Kahangi, E., Coyne, D., & Dubois, T. (2014). Non- pathogenic Fusarium oxysporum endophytes provide field control of nematodes, improving yield of banana (Musasp.). Biological Control, 74, 82–88.

    Article  Google Scholar 

  9. Raaijmakers, J. M., Bruijn, I. D., Nybroe, O., & Ongena, M. (2010). Natural functions of lipopeptides from Bacillus and Pseudomonas: more than surfactants and antibiotics. FEMS Microbiology Reviews, 34, 1037–1062.

    Article  CAS  Google Scholar 

  10. Chen, L., Wang, N., & Wanga, X. (2010). Characterization of two anti-fungal lipopeptides produced by Bacillus amyloliquefaciens SH-B10. Bioresource Technology, 101, 8822–8827.

    Article  CAS  Google Scholar 

  11. Ongena, M., & Jacques, P. (2008). Bacillus lipopeptides: versatile weapons for plant disease biocontrol. Trends in Microbiology, 16, 115–125.

    Article  CAS  Google Scholar 

  12. Arrebola, E., Jacobs, R., & Korsten, L. (2010). Iturin A is the principal inhibitor in the biocontrol activity of Bacillus amyloliquefaciens PPCB004 against postharvest fungal pathogens. Journal of Applied Microbiology, 108, 386–395.

    Article  CAS  Google Scholar 

  13. Yu, G. Y., Sinclair, J. B., Hartman, G. L., & Bertagnolli, B. L. (2002). Production of iturin A by Bacillus amyloliquefaciens suppressing Rhizoctonia solani. Soil Biology and Biochemistry, 34, 955–963.

    Article  CAS  Google Scholar 

  14. Lee, H., Churey, J., & Worobo, R. (2008). Purification and structural characterization of bacillomycin F produced by a bacterial honey isolate active against Byssochlamys fulva H25. Journal of Applied Microbiology, 105(3), 663–673.

    Article  CAS  Google Scholar 

  15. Chen, H., Wang, L., Su, C., Gong, G., Wang, P., & Yu, Z. (2008). Isolation and characterization of lipopeptide antibiotics produced by Bacillus subtilis. Letters in Applied Microbiology, 47, 180–186.

    Article  CAS  Google Scholar 

  16. Vater, J., Kablitz, B., Wilde, C., Franke, P., Mehta, N., & Cameotra, S. S. (2002). Matrix-assisted laser desorption ionization-time of flight mass spectrometry of lipopeptide biosurfactants in whole cells and culture filtrates of Bacillus subtilis C-1 isolated from petroleum sludge. Applied and Environmental Microbiology, 68, 6210–6219.

    Article  CAS  Google Scholar 

  17. Zhang, B., Dong, C., Shang, Q., Han, Y., & Li, P. (2013). New insights into membrane permeabilization of fungal hyphae by the lipopeptide antibiotic bacillomycin L. Biochim Biophys Acta Biomembr, 1828, 2230–2237.

    Article  CAS  Google Scholar 

  18. Qin, Y., Han, Y., Shang, Q., & Li, P. (2015). Complete genome sequence of Bacillus amyloliquefaciens L-H15, a plant growth promoting rhizobacteria isolated from cucumber seedling substrate. Journal of Biotechnology, 200, 59–60.

    Article  CAS  Google Scholar 

  19. Zhang, B., Dong, C., Shang, Q., Cong, Y., Kong, W., & Li, P. (2013b). Purification and partial characterization of Bacillomycin L Produced by Bacillus amyloliquefaciens K103 from Lemon. Applied Biochemistry and Biotechnology, 171, 2262–2272.

    Article  CAS  Google Scholar 

Download references

Acknowledgments

This study was supported by National Natural Science Foundation (No. 31172001), Special Fund for Agro-scientific Research in the Public Interest (No.201303014), the Southwest University PhD Funds to Yuzhu Han (No. 2013Bsr06), and Fundamental Research Funds for the Central Universities (XDJK2015C121).

Author information

Authors and Affiliations

  1. College of Food Science and Nutritional Engineering, China Agricultural University, Room 410, East District, 17# Qinghua East Road, Beijing, 100083, China

    Yuzhu Han, Bao Zhang, Chengzhen You & Pinglan Li

  2. Key Laboratory of Biology and Genetic Improvement of Horticultural Crops, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Room 321, Beijing, 100081, China

    Yaqiong Yu & Qingmao Shang

  3. Department of Animal Science, Southwest University, Rongchang Campus, Chongqing, 402460, China

    Yuzhu Han

  4. Department of Microbiology, The Ohio State University, Columbus, OH, 43210, USA

    Qian Shen

Authors
  1. Yuzhu Han
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Bao Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Qian Shen
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Chengzhen You
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Yaqiong Yu
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Pinglan Li
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Qingmao Shang
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding authors

Correspondence to Pinglan Li or Qingmao Shang.

Additional information

Highlights

Characterization of two antifungal cyclic peptides produced by Bacillus amyloliquefaciens L-H15

1. Two antifungal compounds from B. amyloliquefaciens were purified and identified.

2. A novel cyclic peptide with the molecular weight 852.4 Da was firstly discovered.

3. Another antifungal cyclic peptide was identified as Iturin A with C15β-amino fatty.

4. Both compounds showed inhibitory activity against soil-borne fungal pathogens.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Han, Y., Zhang, B., Shen, Q. et al. Purification and Identification of Two Antifungal Cyclic Peptides Produced by Bacillus amyloliquefaciens L-H15. Appl Biochem Biotechnol 176, 2202–2212 (2015). https://doi.org/10.1007/s12010-015-1708-x

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12010-015-1708-x

Keywords

Search

Navigation